Magnetic analyte collecting and transferring system and method thereof

Abstract

The invention discloses a magnetic analyte collecting and transferring system, which comprises a collecting part and an elution, wherein the collecting part comprises a magnetic bar and a container suitable for placing the magnetic bar in size; the magnetic bar comprises a magnetic core and a magnetic sleeve; the magnetic sleeve is suitable for detachably sleeving the outside of the magnetic core in size; and the elution part is provided with a magnet for attracting magnetic analyte to fall off from the magnetic sleeve. The invention relates to the field of full-automatic detection equipment of the analyte or an extract. The magnetic bar in the system has a magnetic sleeve structure and can be matched with the magnet of the elution part, so that the problem that a common electromagnet or permanent magnet is difficult to separate after adsorption is completed is solved; a reading error generated in the CTC tumor cell metastasis process is reduced; and meanwhile, the pollution of a buffer solution and an antibody magnetic bead on the magnetic core is avoided.

Description

Technical field [0001] The invention relates to the field of automatic detection equipment for analytes or extracts, and particularly provides a system for collecting and transferring magnetic analytes and a method of use thereof. Background technique [0002] Circulating tumor cell (CTC) refers to cancer cells that fall off the tumor and enter the circulatory system. They can fall off from solid tumor lesions (primary or metastatic lesions) spontaneously or through diagnosis and treatment operations, and enter the systemic circulatory system (such as Peripheral blood). Most CTCs will undergo apoptosis or be swallowed after entering the peripheral blood, and a few can escape and anchor to tissues or organs other than the lesion, and then develop into metastases, increasing the risk of death in patients with malignant tumors. These cells can be found through simple blood tests, and can be used as biological markers of cancer, indicating the presence of tumors in the body, their g...

AI Technical Summary

Problems solved by technology

At present, the circulating tumor cell enrichment equipment that has been listed in China is the cellsearch system and ctcbiopsy system (non-magnetic capture system, using physical filtration) of Johnson & Johnson. The cellsearch system uses a magnetic frame to capture circulating tumor cells, and the transfer step requires Pipetting and transferring after demagnetization of the magnet may easily cause liquid residue or adsorption leading to the loss of circulating tumor cells

In addition, the magnetic core is usually in direct contact with the buffer solution during elution, and the solution causes contamination

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