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Epoxidase-2 and application of inhibitor thereof for bladder cancer

A cyclooxygenase and inhibitor technology, applied in the field of medicine, can solve problems such as the undiscovered cyclooxygenase-2 and reports on the role of bladder cancer

Active Publication Date: 2017-05-31
深圳市俊元生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 2-Aminobenzoxazole and its derivatives are typical heterocyclic compounds with diverse activities, but no reports have been found on their effects on cyclooxygenase-2 and bladder cancer

Method used

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  • Epoxidase-2 and application of inhibitor thereof for bladder cancer
  • Epoxidase-2 and application of inhibitor thereof for bladder cancer
  • Epoxidase-2 and application of inhibitor thereof for bladder cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1: Effect of Nimesulide on Cyclooxygenase-2 Expression and Cell Proliferation of Bladder Cancer Cells

[0022] 1. Experimental materials

[0023] Bladder cancer cell line T24 was preserved in our company for a long time; methylthazolium blue MTT, dimethyl sulfoxide DMSO, DMEM medium and fetal bovine serum were purchased from Sigma company. Others are conventional reagents.

[0024] 2. Experimental method

[0025] 1. Cell culture and experimental grouping

[0026] Bladder cancer cell line T24 was cultured according to conventional methods. DMEM medium containing 10% fetal bovine serum, at 37°C, 5% CO 2 And cultured under saturated humidity, change the medium once every 48h. Cells in the logarithmic growth phase were used for the experiment. The experiment was divided into control group and nimesulide group, the control group was not added COX-2 inhibitor, and the nimesulide group was treated with nimesulide. details as follows:

[0027] The bladder cancer...

Embodiment 2

[0044] Example 2: Screening of cyclooxygenase-2 inhibitors

[0045] 1. Experimental materials

[0046] Bladder cancer cell line T24 was preserved in our company for a long time; methylthazolium blue MTT, dimethyl sulfoxide DMSO, DMEM medium and fetal bovine serum were purchased from Sigma company. Others are conventional reagents.

[0047] 2. Experimental method

[0048] 1. Cell culture and experimental grouping

[0049] Bladder cancer cell line T24 was cultured according to conventional methods. DMEM medium containing 10% fetal bovine serum, at 37°C, 5% CO 2 And cultured under saturated humidity, change the medium once every 48h. Cells in the logarithmic growth phase were used for the experiment. The experiment was divided into a control group and a treatment group. The control group was not added with cyclooxygenase-2 inhibitors, and the treatment group was treated with different compounds to be tested. details as follows:

[0050] The bladder cancer cell line T24 in...

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Abstract

The invention discloses an epoxidase-2 and an application of an inhibitor thereof for bladder cancer. According to the invention, the selective inhibitor nimesulide of epoxidase-2 can restrain the multiplication of bladder cancer cells T24 by reducing the expression of the epoxidase-2. A model is adopted for screening and acquiring four epoxidase-2 inhibitors. The four inhibitor compounds are all 2-amido benzoxazole derivative, all can reduce the expression of the epoxidase-2 and can restrain the multiplication of T24. The invention proves that epoxidase-2 can be used as a drug target for screening the drug for restraining the bladder cancer.

Description

technical field [0001] The invention belongs to medicine and relates to the discovery of bladder cancer treatment targets and the development of therapeutic drugs, in particular to the application of cyclooxygenase-2 and its inhibitors in bladder cancer. Background technique [0002] Bladder cancer is a common malignant tumor of the urinary system, most of which are transitional epithelial cell carcinomas. According to the statistics of the World Health Organization in 2002, there are about 360,000 new cases in the world every year, and the incidence rate ranks ninth among malignant tumors. Male patients account for about 75% to 80%, and the average age of onset is 70 years old; there are about 150,000 people every year. Dying from bladder cancer ranks 12th among the deadly cancers, and the natural survival period is about 16 to 20 months without treatment. Clinically, intermittent painless hematuria is the earliest symptom of bladder cancer. Well-differentiated papillary t...

Claims

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Application Information

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IPC IPC(8): A61K38/46A61K45/06A61K31/5377A61K31/454A61K31/423G01N21/31A61P35/00
CPCA61K31/423A61K31/454A61K31/5377A61K38/46A61K45/06C12Y303/02003G01N21/31
Inventor 王玉梅李会
Owner 深圳市俊元生物科技有限公司
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