Small size shaking flask fermentation equipment

Abstract

The invention specially discloses small size shaking flask fermentation equipment which comprises a fermentation equipment main body, wherein the fermentation equipment main body is in a columnar shape; an opening is formed at one end of the fermentation equipment main body; screw threads are formed inside and outside the opening end; a sealed bottom is arranged at the other end of the fermentation equipment main body; the opening end is movably connected with a columnar centrifugal tube through the screw threads; an external screw thread is formed at an opening end of the columnar centrifugal tube; one or more of columnar tubes or square tubes which are vertical to the fermentation equipment main body or are obliquely inserted into the fermentation equipment main body is / are arranged at a columnar part of the fermentation equipment main body; and the columnar tubes or the square tubes are in seamless integration with the fermentation equipment main body to form a connection body. The small size shaking flask fermentation equipment has the advantages that complex sterile operations such as sample taking, sample adding, even medium replacement and the like can be conveniently and fast realized; by selecting different sizes of the lateral openings of the fermentation equipment main body, edges of the top ends of the openings and flask sealing membranes, the oxygen supply quantity in a cultivation process can be adjusted, so as to meet different cultivation conditions of anaerobic cultivation, facultative anaerobic cultivation and aerobiotic cultivation.

Description

technical field [0001] The invention relates to the technical field of fermentation equipment, and specifically discloses a small shake flask fermentation equipment. Background technique [0002] Laboratory-scale small-scale fermentation experiments are indispensable in the experimental exploration of the expression level screening of engineering strains, the optimization of strain induction expression conditions, and the determination and optimization of fermentation processes. At present, these tests are all completed by Erlenmeyer flask shake flask culture. If some tests need to change the medium during the strain culture process, it is realized by the cooperation of shake flask and centrifuge tube. This has the following problems: 1. Due to the size of the shaker flask and the size of the shaker, the test cannot be carried out at high throughput; 2. The sampling and adding of samples during the cultivation cannot be carried out quickly and easily; 3. If necessary To rep...

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Problems solved by technology

This has the following problems: 1. Due to the size of the shaker flask and the size of the shaker, the test cannot be carried out at high throughput; 2. The sampling and adding of samples during the cultivation cannot be carried out quickly and easily; 3. If necessary To replace the culture medium during cultivation, it must be completed with a sterile centrifuge tube. There are many operating steps, which increases the possibility of contamination; 4. During the cultivation process, it is impossible to subjectively adjust the supply mode and amount of oxygen

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