Method for preventing vegetable aphid by means of Harmonia aryridis (Pallas)
A technology for ladybugs and aphids, applied in the field of crop protection, can solve problems such as pesticide residues, vegetable quality reduction, environmental pollution, etc., and achieve long-lasting effects, obvious effects, and wide application prospects
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Embodiment 1
[0028] Embodiment 1, utilize the research of Heterochromia control broad bean aphid
[0029] 1. Materials and methods
[0030] The plant used in this embodiment is broad bean, and it starts to test when it grows 2 true leaves, and puts it in a small sarong (length × width × height is 60cm × 60cm × 60cm); the aphid is the bean aphid (Aphis craccivora Koch); Eggs, 1-2 instar larvae, 3-4 instar larvae, and adults of Harmonia aryridis (Pallas). The 1-2 instar larvae refer to 1st instar larvae and 2nd instar larvae; the 3-4 instar larvae refer to 3rd instar larvae and 4th instar larvae.
[0031] This embodiment is carried out in a constant temperature room under natural light conditions with a temperature of 25 ± 2° C., a relative humidity of 70-80%.
[0032] Release eggs of ladybug Heterochromia: After the broad bean plants grow to 2 true leaves, inoculate 80 adult bean aphids on the shoots of each broad bean plant, and then inoculate the true leaves of each broad bean plant wit...
Embodiment 2
[0058] Embodiment 2, utilize heterochromia ladybug to control greenhouse pepper aphid
[0059] 1. Experimental materials
[0060] Matrine reagent: the active ingredient content is 6%, the dosage form is water, purchased from Inner Mongolia Qingyuanbao Biotechnology Co., Ltd.;
[0061] Harmonia aryridis (Pallas): Harmonia aryridis (Pallas) egg card, 20 eggs per card, purchased from Beijing Kuoye Tianyuan Biotechnology Co., Ltd.;
[0062] Test crop: Pepper in the early flowering stage under normal water and fertilizer management.
[0063] 2. Experimental method
[0064] Experimental environment: greenhouse, temperature 18-31°C, relative humidity 55-75%;
[0065] Experimental location: Yanqing, Beijing;
[0066] Control object: Aphids, mainly Myzus persicae (Suler) which damage pepper.
[0067] Three treatments were set up in the experiment: matrine, release of ladybug eggs and blank control. Each treatment was repeated 3 times, and each repetition was a plot. A total of 9 p...
Embodiment 3
[0082] Embodiment 3, utilize heterochromia ladybug to control greenhouse sweet pepper aphid
[0083] 1. Experimental materials
[0084] Harmonia aryridis (Pallas): Harmonia aryridis (Pallas) egg card, 20 eggs per card, purchased from Beijing Kuoye Tianyuan Biotechnology Co., Ltd.;
[0085] Test crops: sweet peppers in flowering and fruit-setting period under normal water and fertilizer management.
[0086] 2. Experimental method
[0087] Experimental environment: Greenhouse, temperature 22-35°C, relative humidity 50-75%;
[0088] Experimental location: Changping, Beijing;
[0089] Control object: Aphids, mainly Myzus persicae (Suler) that damage sweet peppers.
[0090] Two treatments were set up in the experiment: release of ladybug eggs and blank control. Each treatment was repeated 3 times, and each repetition was a plot. A total of 6 plots were set up, and a random block design was adopted. Each plot has an area of 12 square meters and 30 to 32 bell peppers. Two iso...
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