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A fluorescent nanometer thermometer based on silver nanowires and its preparation method

A technology of silver nanowires and fluorescent nanometers, applied to thermometers, thermometers, instruments, etc. that change physically/chemically, can solve the problems of unsatisfactory temperature measurement and low spatial resolution, and achieve easy surface functionalization and simple preparation , good quenching effect

Active Publication Date: 2019-04-05
TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Commonly used thermometers (such as thermocouple thermometers) are unable to measure the temperature in small systems (such as specific regions in cells) due to low spatial resolution.

Method used

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  • A fluorescent nanometer thermometer based on silver nanowires and its preparation method
  • A fluorescent nanometer thermometer based on silver nanowires and its preparation method
  • A fluorescent nanometer thermometer based on silver nanowires and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1 is the preparation method of the fluorescent nano-thermometer based on silver nanowire

[0033] (1) Preparation of silver nanowires by polyol method

[0034] a. Prepare 10ml 0.1M AgNO 3 solution, 9ml 0.15M PVP solution, 1ml 0.3mM FeCl 3 Solution, above solvent is ethylene glycol;

[0035] b. Add 1ml 0.3mM FeCl 3 The solution was added to 9ml 0.15M PVP solution, mixed evenly and then added dropwise to vigorously stirred 10ml 0.1M AgNO 3 After the reaction is cloudy, stop stirring (about 4h) and transfer to an autoclave to keep at 160°C for 3 hours; take out the product, wash it with acetone, ethanol and water respectively, and store it in alcohol after washing. The scanning electron micrographs of the prepared silver nanowires are as follows: figure 2 shown.

[0036] (2) Specific DNA fragments (ATCTAATCATTATTGTTTTTTTTTTTTTTTTACTATTATGTTTAGATTTTTTTTTTT-(CH 2 ) 3 -SH) (20 μL, 100 μM) and accessory chain T (ie TTTTTTTTTT-(CH 2 ) 3-SH) (10 μL, 100 μM)...

Embodiment 2

[0038] Embodiment 2 is the preparation method of the fluorescent nano-thermometer based on silver nanowire

[0039] (1) Silver nanowires were prepared by polyol method.

[0040] a. Prepare 10ml 0.1M AgNO 3 solution, 9ml 0.15M PVP solution, 1ml 0.3mM FeCl 3 Solution, above solvent is ethylene glycol;

[0041] b. Add 1ml 0.3mM FeCl 3 The solution was added to 9ml 0.15M PVP solution, mixed evenly and then added dropwise to vigorously stirred 10ml 0.1M AgNO 3 in the solution; after the reaction is turbid, stop stirring (about 4h) and transfer to an autoclave to keep at 160°C for 3 hours; take out the product, wash it with acetone, ethanol and water respectively, and store it in alcohol after washing;

[0042] (2) Specific DNA fragments (ATCTAATCATTATTGTTTTTTTTTTTTTTTTACTATTATGTTTAGATTTTTTTTTTT-(CH 2 ) 3 -SH) (20 μL, 100 μM) and accessory chain T (ie TTTTTTTTTT-(CH 2 ) 3 -SH) (10 μL, 100 μM) was mixed with tris(2-formylethyl)phosphine hydrochloride acetate buffer (PH=5.2) (...

Embodiment 3

[0044] Embodiment 3 is the preparation method of the fluorescent nano-thermometer based on silver nanowire

[0045] (1) Silver nanowires were prepared by polyol method.

[0046] a. Prepare 10ml 0.1M AgNO 3 solution, 9ml 0.15M PVP solution, 1ml 0.3mM FeCl 3 Solution, above solvent is ethylene glycol;

[0047] b. Add 1ml 0.3mM FeCl 3 The solution was added to 9ml 0.15M PVP solution, mixed evenly and then added dropwise to vigorously stirred 10ml 0.1M AgNO 3 in the solution; after the reaction is turbid, stop stirring (about 4h) and transfer to an autoclave to keep at 160°C for 3 hours; take out the product, wash it with acetone, ethanol and water respectively, and store it in alcohol after washing;

[0048] (2) Specific DNA fragments (ATCTAATCATTATTGTTTTTTTTTTTTTTTTACTATTATGTTTAGATTTTTTTTTTT-(CH 2 ) 3 -SH) (20 μL, 100 μM) and accessory chain T (ie TTTTTTTTTT-(CH 2 ) 3 -SH) (10 μL, 100 μM) mixed with tris(2-formylethyl)phosphine hydrochloride acetate buffer (PH=5.2) (30 μ...

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Abstract

The present invention discloses a silver nanowire-base fluorescent nanometer thermometer. According to the thermometer, a silver nanowire is adopted as a base; the surface of the silver nanowire is provided with a one-dimensional nanometer thermometer provided with a specific DNA fragment with fluorescent molecules, and an auxiliary chain T; the sequence of the specific DNA fragment is ATCTAATCATTATTGTTTTTTTTTTTTTTTACTATTATGTTTAGATTTTTTTTTTT of which the 3' end is modified with -(CH2)3-SH; and the fluorescent molecules is 5'TEXAS red; and the sequence of the auxiliary chain T is TTTTTTTTTT of which the 3' end is modified with -(CH2)3-SH. The present invention further discloses a preparation method of the above-mentioned fluorescent nanometer thermometer. According to the silver nanowire-base fluorescent nanometer thermometer and the preparation method thereof of the invention, the silver nanowire is adopted as the base, the fluorescent molecules are assembled on the surface of the silver nanowire through the specific DNA fragment which can respond to temperature, and therefore, the silver nanowire-base fluorescent nanometer thermometer can be constructed. The thermometer can be used for detecting the temperature of tiny systems.

Description

technical field [0001] The invention relates to the field of nano thermometers. More specifically, it relates to a fluorescent nanometer thermometer based on silver nanowires and a preparation method thereof. Background technique [0002] As an important parameter, temperature plays a regulatory role in many chemical reactions and biological processes. Accurately measuring the temperature of tiny systems is of great significance for regulating microchemical reactions and understanding life processes at the cellular level. Commonly used thermometers (such as thermocouple thermometers) cannot measure the temperature in small systems (such as specific regions in cells) due to problems such as low spatial resolution. With the improvement of nanomaterial preparation methods and the continuous development of nanotechnology, nanometer thermometers have been widely studied (Qi, Li. Surface-Modified Silicon Nanoparticles with Ultrabright Photoluminescence and Single-Exponential Dec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01K11/00
CPCG01K11/006
Inventor 穆丽璇卜聪聪师文生
Owner TECHNICAL INST OF PHYSICS & CHEMISTRY - CHINESE ACAD OF SCI
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