Liver injury and repair type active peptide prepared from tuna offal
A technology of repairing effect and leftovers, applied in the field of active peptides, can solve problems such as the effect and safety of lipid-lowering drugs that need to be studied, and achieve the effects of increasing value, protecting the environment, and avoiding waste
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[0024] 1. Method
[0025] 1.1. Pretreatment of tuna scraps
[0026] Take the fresh tuna leftovers and put the fish meat into distilled water, stir gently, wash away the impurities, homogenate, and store it at -20°C for later use.
[0027] 1.2. Screening of the best enzymes for enzymatic hydrolysis of tuna leftovers
[0028] Using alkaline protease, pepsin, neutral protease, trypsin and papain, the optimum temperature and optimum pH were selected respectively, the enzymolysis time was 6 hours, and the amount of enzyme added was 1000U / g. Carry out enzymatic hydrolysis, enzymatic hydrolysis conditions are as shown in table 1. After the enzymolysis, the enzyme activity was inactivated at 100°C for 10 minutes, and then freeze-dried for later use. The dried product was applied to the model group cells at a concentration of 10 mg / mL for 24 hours, and the protease that reduced the TG content of the model group cells the most was screened out. . The calculation method of TG content...
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