Pollen collecting method for tobacco hybrid seed production

Abstract

The invention provides a pollen collecting method for tobacco hybrid seed production, and belongs to the technical field of the tobacco improved variety breeding. The method comprises the following steps: picking flowers that the growth stages are consistent, the corollas will be opened, and the top ends are light red and inflated in the male parent field, threshing by using an anther thresher, separating the anther from the impurities, such as filaments, stigmas and petals by using 20 meshes of a fine sieve, collecting the anther, placing in a constant temperature drying cabinet and drying, taking the anthers after the anther is completely cracked and the pollen is completely dispersed, separating the pollen from pollen sac by using 60 meshes of a sample sieve, collecting the pollen, loading in a wild-mouth bottle, and placing in a dryer loaded with a drying agent, storing in a freezer for future use in low temperature. The pollen collecting method is capable of reducing the male and female parent planting proportion, solving the problem that the flowering phases of the male and female parents in the tobacco hybrid seed production are asynchronous, reducing the labor force for the tobacco hybrid seed production, reducing the cost of the hybrid seed production, and improving the efficiency of the hybrid seed production.

Description

technical field [0001] The invention relates to a pollen collection method for tobacco hybrid seed production, belonging to the technical field of tobacco breeding. Background technique [0002] In the process of traditional tobacco hybrid seed production, artificial pollination is a time-consuming and labor-intensive task. Before pollination, pollen must be stripped from the male parent anthers for pollination, which is usually picked and stripped now. Picking a male parent flower can only pollinate 5-10 female parents. It is often necessary to plant a large number of male parents and invest a lot of manpower to collect pollen to meet the pollination needs of the female parent, resulting in low pollination efficiency and high seed production costs. Bottlenecks limiting the development of tobacco hybrid seed production. Contents of the invention [0003] The invention provides a pollen collection method for tobacco hybrid seed production. By adopting the method, the effic...

AI Technical Summary

Problems solved by technology

Picking a male parent flower can only pollinate 5-10 female parents. It is often necessary to plant a large number of male parents and invest a lot of manpower to collect pollen to meet the pollination needs of the female parent, resulting in low pollination efficiency and high seed production costs. The Bottleneck Limiting the Development of Tobacco Hybrid Seed Production