Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Compound typing system used for personal identification

A technology of genetic markers and loci, applied in the field of forensic identification, can solve problems such as unsatisfactory typing and identification results, and inability to make satisfactory family identification conclusions

Active Publication Date: 2017-03-22
INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA +1
View PDF5 Cites 11 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although many genetic marker typing systems for identification and individual identification have been developed, for some relatives with distant ancestry, the existing genetic marker composite typing system cannot make a satisfactory conclusion on family identity
For example, in practical applications, the existing typing system is not ideal for the typing and identification of some relatives such as grandchildren, uncles, or half-siblings, and the identification of such genetic relationships is also a problem that is often faced in practical applications

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Compound typing system used for personal identification
  • Compound typing system used for personal identification
  • Compound typing system used for personal identification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1 1

[0053] Example 1 A group of genetic marker combinations

[0054] The genetic marker combination includes 482 SNP sites described in the present invention, and the rs numbers and genetic information of the 482 SNP sites are shown in Table 1.

Embodiment 2

[0055] Embodiment 2 A kind of typing kit

[0056] This typing kit contains:

[0057] (1), sequencing library preparation reagents; (2), high-throughput sequencing reagents.

Embodiment 1

[0059] A random sample experiment and result analysis were performed on family members with 16 relatives.

[0060] Individual samples: a total of 47 family members of 16 relatives, including parents, children, brothers, sisters, cousins, grandparents and nephews; blood samples were used as samples.

[0061] experiment procedure:

[0062] Venous blood collection was performed on the above 47 individuals, and 47 blood samples were obtained, which were randomly numbered and double-blind experiments were carried out:

[0063] (1), DNA extraction:

[0064] Use GenElute TM The blood genomic DNA kit extracted genomic DNA from 47 blood samples respectively, and obtained 47 blood genomic DNA samples. For the specific extraction process, refer to the kit instructions;

[0065] (2), library preparation:

[0066] A. PCR amplification:

[0067] Using the 47 blood genomic DNA samples obtained in step (1) of this embodiment as PCR templates, primers were designed, and Ion AmpliSeq was u...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention aims at providing a compound typing system used for personal identification, which is used for personal identification, especially the identification for relative relations such as grandparents and grandchild, uncles and nephews, or half sibs, and has the powerful typing efficiency and wide application range. On one aspect, the invention provides a set of genetic marker assembly, and the genetic marker assembly comprises 482 SNP sites positioned on the autosome; on another aspect, the invention provides a typing kit, and the typing kit comprises a reagent for typing the 482 SNP sites in the genetic marker assembly provided by the invention; on still another aspect, the invention provides an individual genotype identity card, and the individual genotype identity card comprises a chip, the genotype information of the genetic marker sites of the individuals is stored in the chip, and the genetic marker sites refer to the 482 SNP sites in the genetic marker assembly provided by the invention.

Description

technical field [0001] The invention belongs to the technical field of forensic identification and relates to a compound typing system for identification, in particular to 482 SNP sites and its application in identification. Background technique [0002] Forensic DNA analysis is the application of modern DNA analysis technology to analyze the distribution and transmission rules of DNA genetic markers in the population, to determine the consistency and kinship of the analyzed samples, so as to identify the source samples of the samples, and to provide services for the investigation and judicial trial. Technical Support. Its analysis objects mainly involve various body fluids (spots), tissues, hair and epidermal exfoliated cells of the human body. At present, the technologies used for identification in forensic DNA analysis are mainly for short tandem repeats (Short tandem repeats, STR) and single nucleotide polymorphisms (Single nucleotide polymorphisms, SNP) typing. [000...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/156
Inventor 倪铭莫少康严江伟伯晓晨
Owner INST OF RADIATION MEDICINE ACAD OF MILITARY MEDICAL SCI OF THE PLA
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com