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A method for simultaneous determination of glutathione and free amino acids in shellfish

A technology of free amino acid and glutathione, which is applied in the field of simultaneous determination of glutathione and free amino acid in shellfish, can solve the problem that there is no fast and effective method for simultaneous determination, and achieves the advantages of separation, measurement of a wide range of The effect of accurate measurement data

Active Publication Date: 2018-07-27
SHANGHAI OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods mainly measure the content of GSH or FAA alone, and there are relatively few studies on the methods for the determination of GSH or FAA in shellfish. However, the simultaneous determination of GSH and FAA in shellfish has not always been fast and effective. Methods,

Method used

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  • A method for simultaneous determination of glutathione and free amino acids in shellfish
  • A method for simultaneous determination of glutathione and free amino acids in shellfish
  • A method for simultaneous determination of glutathione and free amino acids in shellfish

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040]Preparation of mixed standard solution: Accurately weigh 76.83 mg of glutathione standard substance, dilute it to a 100 ml volumetric flask with ultrapure water to obtain a 2.5 μmol / ml standard solution, accurately take out 2 ml of the mixed solution, add 2 ml2. 5μmol / mL mixed solution containing multiple standard amino acid components, dilute to 50ml with ultrapure water, that is, 0.10μmol / mL mixed standard solution, and store in a refrigerator at 4°C.

[0041] Preparation of buffer:

[0042] (1) Preparation of B1 reagent: Weigh 6.0g of sodium citrate dihydrate, 7ml of 1mol / L sodium hydroxide, 5.66g of sodium chloride, 19.80g of citric acid, and 135.0ml of ethanol, mix thoroughly and dilute to volume with ultrapure water to 1 L at a pH of 3.5;

[0043] (2) Preparation of B2 reagent: Weigh 7.80g of sodium citrate dihydrate, 20ml of 1mol / L sodium hydroxide, 7.07g of sodium chloride, 22.00g of citric acid, and 25.0ml of ethanol, mix thoroughly and dilute to volume with ul...

Embodiment 2

[0052] Preparation of buffer:

[0053] (1) Preparation of B1 reagent: Weigh 6.40g of citric acid dihydrate, 6ml of 1mol / L sodium hydroxide, 5.66g of sodium chloride, 21.50g of citric acid, and 135.0ml of ethanol. 1L, pH 3.1;

[0054] (2) Preparation of B2 reagent: Weigh 7.60g of citric acid dihydrate, 20ml of 1mol / L sodium hydroxide, 7.07g of sodium chloride, 24.00g of citric acid, and 25.0ml of ethanol. 1L, pH 3.0;

[0055] (3) Preparation of B3 reagent: Weigh 13.31g of citric acid dihydrate, 3.74g of sodium chloride, 15.60g of citric acid, and 9.0ml of ethanol, mix well, and dilute to 1L with ultrapure water, with a pH of 4.0;

[0056] (4) Preparation of B4 reagent: Weigh 26.80 g of citric acid dihydrate, 54.35 g of sodium chloride, and 6.30 g of citric acid and mix them thoroughly, then dilute to 1 L with ultrapure water, and the pH is 4.5;

[0057] (5) Preparation of B5 reagent: Weigh 8.0 g of sodium hydroxide and 100.0 ml of ethanol, mix well, and dilute to 1 L with ul...

Embodiment 3

[0063] Take 20 μl of the standard mixed solution obtained in Example 1, use the original reagent and its system parameters, measure it with an automatic amino acid analyzer, perform 3 parallel experiments, and take the average value of the measurement results. Specific test results such as figure 1 .

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Abstract

The invention belongs to field of chemical analysis and detection, and particularly relates to a method for simultaneously measuring glutathione and free amino acid in shellfish. According to the method, pretreatment of samples is optimized, separation resin is protected, separation is facilitated, extraction efficiency of the amino acid is better, antioxidants are added, so that the glutathione cannot be oxidized, home-made buffer solution and reaction solution are used, pH (potential of hydrogen) and elution processes of the buffer solution are changed, peaky overlapping or interaction effect is eliminated, cost is greatly reduced, consumed cost is one tenth of that of original reagents, data are accurately measured, and measuring types are wider.

Description

technical field [0001] The invention belongs to the field of chemical analysis and detection, in particular to a method for simultaneously measuring glutathione and free amino acids in shellfish. Background technique [0002] Shellfish belong to the clambranchia (or bivalves) in the mollusk phylum. Common snails, clams, scallops, oysters, etc. all belong to this category. Shellfish not only have edible value, but also have high medicinal value. Due to the complexity of their living environment, shellfish contain many substances with special physiological activities that are not found in terrestrial organisms. They are rich in non-volatile nitrogen-containing substances such as active peptides and amino acids. According to different types, their bodies The composition and content are also different, which also provides a good foundation for the development and utilization of shellfish resources. Glutathione ((glutathione, GSH)) has been studied by scholars at home and abroad...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N35/00G01N1/28G01N1/34
CPCG01N1/28G01N1/34G01N35/00
Inventor 邱伟强谢晶陈舜胜金银哲张苏平桂娟
Owner SHANGHAI OCEAN UNIV
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