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Method for judging quality of fresh tobacco leaf sample in tobacco metabonomics based on pigments

A technology of fresh tobacco leaves and metabolomics, applied in the field of analytical chemistry, can solve the problem of difficult to accurately determine whether the sample is normal or not, and achieve the effects of accurate and reliable results, less sample consumption, and simple and fast operation.

Active Publication Date: 2017-02-22
ZHENGZHOU TOBACCO RES INST OF CNTC
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AI Technical Summary

Problems solved by technology

However, the color of browned tobacco leaf samples will change from bright green to brown, but for samples with slight enzymatic browning reaction, it is difficult to accurately judge whether the sample is normal or not only by observing the color depth of the sample appearance

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The pigment content in fresh tobacco leaf samples was determined by HPLC, and it was found that the ratio of carotene to chlorophyll b content in browned samples decreased significantly.

[0017] The samples to be tested included 127 fresh tobacco leaf samples from 7 varieties and 20 origins in Fujian, Guizhou, Hunan, Henan, Yunnan and other provinces (each point has 5-7 biological replicates). These include 92 qualified samples (samples were strictly controlled at -80°C during collection, transportation and pretreatment), and 35 browned samples (completely browned due to insufficient liquid nitrogen during storage and transportation).

[0018] The sample analysis steps are as follows:

[0019] 1) Fresh tobacco leaves picked from the field were stored and transported in a liquid nitrogen tank at -196°C, frozen and ground in liquid nitrogen, freeze-dried at low temperature, and stored in a refrigerator at -80°C for subsequent metabolomic analysis. Accurately weigh about...

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Abstract

The invention provides a method for judging the quality of a fresh tobacco leaf sample in the tobacco metabonomics based on pigments. According to the method, the ratio of the content of carotene to the content of chlorophyll b in the fresh tobacco leaf sample is adopted as an index, the quality of the fresh tobacco leaf sample in the metabonomics is judged, and bases are provided for selection of qualified samples in the metabonomics. Compared with the prior art, the method has the advantages of being easy and fast to operate and accurate and reliable in result and adopting fewer samples, and reference can be provided for tobacco metabolic characteristic analysis and relevant tobacco metabonomics study.

Description

technical field [0001] The invention relates to the field of analytical chemistry, in particular to a method for judging whether the quality of fresh tobacco leaf samples in tobacco metabolomics meets the needs of metabolomics research. Background technique [0002] Plant metabolomics is a technique for studying plants by examining the changes of metabolites in the later stages of plant stimulation or disturbance or their changes over time. In the process of metabolomics profiling analysis, the collection of typical samples is the first and extremely important step. Obtaining qualified samples to truly and objectively reflect the metabolic state of the sample is the basis and premise of metabolomics analysis. The collection of fresh plant leaf samples needs to be quenched at low temperature to stop their physiological activities to ensure their metabolic state at the time of sampling. The standard practice is to pick plant samples, wrap them in tin foil, freeze them in -196°...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 陈霞刘萍萍徐国云张慧申晓晔周会娜金立锋翟妞魏攀王晨
Owner ZHENGZHOU TOBACCO RES INST OF CNTC
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