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High-throughput Zika virus nonstructural protease activity assay buffer and activity assay method

A Zika virus, activity assay technology, applied in the field of medicine, to achieve accurate results and improve the effect of activity

Active Publication Date: 2020-04-10
TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although most Zika virus-infected patients have mild symptoms and usually recover on their own after 3-7 days of onset, the medical community generally believes that this fast-spreading virus may be associated with an abnormally high number of newborns with microcephaly

Method used

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  • High-throughput Zika virus nonstructural protease activity assay buffer and activity assay method
  • High-throughput Zika virus nonstructural protease activity assay buffer and activity assay method
  • High-throughput Zika virus nonstructural protease activity assay buffer and activity assay method

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Embodiment 1

[0036] The active assay buffer of high-throughput Zika virus nonstructural protease of the present invention, buffer component comprises: trishydroxymethyl aminomethane 100mM, sodium chloride 20mM, the glycerol that volume fraction is 10%; The pH value of buffer is 8.0 .

[0037] The buffer has the effect of improving the activity of Zika virus non-structural protease.

Embodiment 2

[0039] The active assay buffer of high-throughput Zika virus nonstructural protease of the present invention, buffer composition comprises: Tris 50mM, sodium chloride 10mM, CHAPS 0.5mM, galactose 10mM, volume fraction is the glycerol of 40% ; The pH value of the buffer is 8.5.

[0040] The buffer has the effect of improving the activity of Zika virus non-structural protease.

Embodiment 3

[0042] The activity assay buffer of high-throughput Zika virus nonstructural protease of the present invention, buffer component comprises: trishydroxymethylaminomethane 20mM, sodium chloride 5mM, CHAPS 1mM, galactose 20mM, the glycerol that volume fraction is 15%; The pH of the buffer was 8.7.

[0043] The buffer has the effect of improving the activity of Zika virus non-structural protease.

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Abstract

The invention discloses an activity determination buffer solution and an activity determination method of high-throughput Zika virus non-structural protease, wherein the buffer solution consists of the following buffer ingredients: 10-200mM of trihydroxymethyl aminomethane, 0-20mM of sodium chloride, 0-1mM of CHAPS, 0-20mM of galactose and glycerol which is 10-40% of in volume percentage, and the pH value of the buffer solution is at 8.0-9.0. The buffer solution provided by the invention can improve the activity of the protease in a process of detecting the enzymatic activity of Zika viruses, so that the high-throughput Zika virus non-structural protease activity determination method, which makes use of the buffer solution, is more accurate in result, and subsequently an effect of guiding the screening of Zika virus protease activity inhibitors is achieved and the research and development progress of medicines targeted to the Zika viruses is promoted.

Description

technical field [0001] The invention relates to the technical field of medicines, in particular to a high-throughput Zika virus non-structural protease activity assay buffer and an activity assay method. Background technique [0002] Zika virus (ZIKV) belongs to the Flaviviridae family (Fla.viviridae) and the genus Flavivirus. It is spherical in shape with a diameter of about 40-70 nm and has an envelope. Yellow fever virus belongs to the yellow fever virus genus of the yellow fever family. It is an RNA virus with viscerotropism and neurotropism. It is easy to die at room temperature. The genome is a single-stranded positive-strand RNA with a length of about 10.8Kb. The genome contains an open reading frame (ORF) encoding a polyprotein. [0003] Zika virus was first isolated in 1947 from macaques in the forests of Uganda. It was first isolated from the human body in 1952. As of the end of the last century, Zika virus infection was thought to be distributed only in Africa,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/37
CPCC12Q1/37
Inventor 杨海涛胡灿陈成王泽方蔡岩
Owner TIANJIN INT JOINT ACADEMY OF BIOTECH & MEDICINE
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