A kind of pcr reaction device and using method thereof

Abstract

The invention provides a PCR reaction device. According to the PCR reaction device, a first through hole and a second through hole are formed in a semi-closed channel to realize communication between the PCR reaction device and outside, wherein a reaction system can enter a PCR reaction tube through one of the through holes; the other through hole through which the reaction system does not pass realizes inside-outside gas pressure balance, under a semi-closed state, of the PCR reaction device, so that connection between the PCR reaction device and a reaction device at the front end and conveying of the reaction system are realized. The invention further provides a using method of the PCR reaction device. After a tubular body enters the semi-closed channel, a closing head is pressed to realize charging and discharging of the tubular body, so that the through holes are closed and opened by the tubular body, and therefore, a closing process of the PCR reaction device is simplified; and the closing of the PCR reaction device can be easily realized in manual and mechanical ways. The PCR reaction device provided by the invention is simple in structure, is easy in preparation, can effectively realize semi-closing of the PCR reaction device, is simple and convenient in operation process, and is easy to implement.

Description

technical field [0001] The invention relates to the technical field of PCR reaction devices, in particular to a PCR reaction device and a usage method thereof. Background technique [0002] PCR (Polymerase Chain Reaction, Polymerase Chain Reaction) is a technique for amplifying and amplifying specific DNA (deoxyribonucleic acid, deoxyribonucleic acid) fragments in vitro. When PCR amplifies DNA, it generally needs to go through three steps: high-temperature melting reaction, annealing reaction and extension reaction. After the above three steps, the number of initial DNA molecules doubles, which is a cycle at this time; the doubled DNA molecules then become The template for the next cycle, so that the cycle is multiplied, and after 30-40 cycles, the number of DNA molecules will be amplified to nearly 109 times the initial value. Thus, for the purpose of analysis and inspection, PCR can specifically amplify the DNA molecule as an analyte on a large scale. Therefore, PCR techn...

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Problems solved by technology

[0005] The invention provides a PCR reaction device and its use method to solve the problem that the existing PCR reaction device needs to use external media to complete the input and output of liquid during operation

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