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Monoclonal antibody and preparation method thereof

A monoclonal antibody, nucleotide sequence technology, applied in the field of medicine, can solve the problems of large toxic and side effects, the antibody does not have high affinity, and does not have high specificity

Inactive Publication Date: 2017-02-15
BINZHOU MEDICAL COLLEGE
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  • Summary
  • Abstract
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  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The purpose of the present invention is to provide a monoclonal antibody and its preparation method, aiming to solve the problem that the prior art antibody does not have high affinity, no high specificity, large toxic and side effects, and the concentration of the recombinant antibody obtained in the prior art preparation method is too low. Low cost, high cost, unsuitable for industrial mass production applications

Method used

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  • Monoclonal antibody and preparation method thereof
  • Monoclonal antibody and preparation method thereof
  • Monoclonal antibody and preparation method thereof

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preparation example Construction

[0113] Such as figure 1 Shown: the preparation method of the monoclonal antibody provided by the embodiment of the present invention comprises the following steps:

[0114] S101: immunize mice with PGAM, CoIα, Trx, PGAM13, CoIα12, Trx11 by intrasplenic embedding;

[0115] S102: Fusion of splenocytes from immunized mice with mouse myeloma cells SP2 / 0;

[0116] S103: Detect the cell culture supernatant by indirect ELISA.

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Abstract

The invention discloses a monoclonal antibody and a preparation method thereof. The monoclonal antibody comprises anti-PGAM, CoI alpha, Trx, PGAM 13, CoI alpha 12 and Trx 11. The preparation method comprises the following steps: immunizing mice by embedding PGAM, CoI alpha, Trx, PGAM 13, CoI alpha 2 and Trx 11 in spleens; fusing spleen cells of the mice with myeloma cells SP2 / 0 of the mice; and detecting the cells by indirect ELISA and culturing supernatant. 3, 2, 5, 3, 4 and 3 strains of monoclonal antibodies for resisting PGAM, CoI alpha, Trx, PGAM 13, CoI alpha 12 and Trx 11 are respectively obtained, and homotypes of the antibodies are IgM.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a monoclonal antibody and a preparation method thereof. Background technique [0002] The main function of antibodies is to combine with antigens (including foreign and self), so as to effectively remove foreign substances such as microorganisms and parasites invading the body, especially monoclonal antibodies, because of their high uniformity and specific antigen expression. Therefore, it is widely used in the fields of immunological analysis, radioimmunoimaging and immune-guided therapy. The development of monoclonal antibodies has gone through four stages, namely: murine monoclonal antibodies, chimeric monoclonal antibodies, humanized monoclonal antibodies and fully human monoclonal antibodies. Mouse-derived monoclonal antibody: mouse hybridoma monoclonal antibody mainly fuses B cells from immunized mice with myeloma cells, and then screens out mouse hybrid fusio...

Claims

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Application Information

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IPC IPC(8): C07K16/00C12N15/13
CPCC07K16/00
Inventor 吴玉龙程梅刘士霞周秀芝张莹张玉梅耿丽李波清金昌洙
Owner BINZHOU MEDICAL COLLEGE
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