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A kit for screening colorectal cancer using common small molecules

A colorectal cancer, small molecule technology, applied in the biological field, can solve problems such as instability of lipid molecules

Active Publication Date: 2018-01-19
大连润生康泰医学检验实验室有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the instability of lipid molecules, it has high requirements for clinical sampling, sample processing and subsequent analysis

Method used

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  • A kit for screening colorectal cancer using common small molecules
  • A kit for screening colorectal cancer using common small molecules
  • A kit for screening colorectal cancer using common small molecules

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Example 1. Construction process of the kit for screening colorectal cancer

[0020] 1) Research objects and sample collection

[0021] All samples were collected from May 2013 to May 2015. The samples were from the First Affiliated Hospital of Jinzhou Medical University, Jinzhou Central Hospital, Zhongshan Hospital Affiliated to Dalian University, and Liaoning Cancer Hospital. After taking blood from fasting patients in the morning, one part was used for biochemical detection, and the other part was used for tandem mass spectrometry detection. Blood used for biochemical testing was used directly for testing. The blood used for mass spectrometry detection was immediately dropped into the collection circle of the dried blood sheet collection card to prepare a dried blood sheet, which was dried and stored in a sealed bag, and stored uniformly in a refrigerator at -80 degrees Celsius. This project was approved by the Institutional Review Board, and all subjects signed inf...

Embodiment 2

[0043] Example 2 Application of the kit for screening colorectal cancer

[0044] Take the blood of 50 non-colorectal cancer controls (all clinically identified, including healthy volunteers) and 100 colorectal cancer patients (all clinically identified and diagnosed), and detect C16:1-OH according to the method in Example 1 , Na, C5DC / C16, C10:2 / C10, Glu, Lys, Trp, C4 / C3 content. Substitute the content values ​​of these indicators into the regression model formula to calculate, and draw and mark on the comparison card of the standard kit.

[0045] The results are shown in the figure, each CRC patient and control group can be clearly identified, the straight line in the figure is Y=0.5, the upper side of the straight line is judged as colorectal cancer patients, and the lower side is judged as non-colorectal cancer. After analysis, a total of 110 samples were on the upper side of the straight line, among which 100 colorectal cancer patients were all correctly diagnosed (all we...

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Abstract

The invention discloses a kit for screening colorectal cancer by using common small molecules, and belongs to the field of biotechnology. The kit adopts common small molecules C16:1-OH, Na, C5DC / C16, C10:2 / C10, Glu, Lys, Trp and C4 / C3 as colorectal cancer diagnosis molecular markers, molecule detection models corresponding to the common small molecules are established, and the sensitivity and specificity of colorectal cancer detection are respectively 90.9% and 100%. By adopting the indexes, a remarkable differentiation effect for colorectal cancer and normal references can be achieved, the screening process is accurate, rapid, simple and convenient, and the kit is prior to markers which are used in recent years to study colorectal cancer.

Description

technical field [0001] The invention relates to a kit for screening colorectal cancer by using common small molecules, which belongs to the field of biotechnology. Background technique [0002] Colorectal cancer, including rectal cancer and colon cancer, is one of the most common malignant tumors. Developed countries in Western Europe and North America are the countries with the highest incidence, and their incidence and mortality rank second among all malignant tumors. Relevant data show that colorectal cancer ranks 4th to 6th among malignant tumors in my country, and there is a gradual upward trend. According to the epidemiological statistics of Liaoning Province, compared with 10 years ago, the incidence of colorectal cancer has increased by more than 10%. Although the treatment methods are becoming more and more perfect, the overall survival of patients has not improved significantly. [0003] Judging from the current level of medical development technology, early diag...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/50G01N30/02G01N30/72
CPCG01N30/02G01N30/72G01N33/5002
Inventor 孙宏治曹云峰朱志图房中则高鹏孙晓宇洪沫葛广波尹沛源赵硕杨重恒董军
Owner 大连润生康泰医学检验实验室有限公司
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