Fast and efficient saliva DNA extraction kit and extraction method

Abstract

The invention discloses a fast and efficient saliva DNA extraction kit and an extraction method. The fast and efficient saliva DNA extraction kit contains cell lysis buffer, DNA combined liquid, rinsing liquid and eluant. The extraction method of saliva DNA at least comprises the following three steps: (1) cell lysis: adding the cell lysis buffer into saliva, thereby obtaining the cell lysis buffer containing free DNA; (2) adding DNA combined liquid into the cell lysis buffer obtained in the step (1), thereby obtaining a DNA-magnetic bead absorbing compound; (3) utilizing rinsing liquid and eluant to elute the compound generated in the step (2), and removing RNA and other impurities, thereby obtaining high-purity DNA. The novel cell lysis buffer is adopted by the fast and efficient saliva DNA extraction kit; the cost is low; the extracted DNA purity is high; the completeness is excellent; no RNA is remained; the fast and efficient saliva DNA extraction kit can be applied to high-throughput sequencing, genetic typing and general molecular biological experiment operation.

Description

technical field [0001] The invention belongs to the field of molecular biology, in particular to a fast and efficient saliva DNA extraction kit and extraction method. Background technique [0002] DNA extraction and purification is a basic operation in molecular biology research, and obtaining high-quality DNA is very important for subsequent research and analysis. High-quality genomic DNA is required for human disease detection, molecular diagnosis, and genetic analysis. At present, the DNA used for detection in laboratory departments is generally extracted from anticoagulated blood, and it is not common to extract DNA from saliva for genetic testing. [0003] The main reasons for extracting DNA from saliva are as follows: blood collection usually requires professional equipment and professionals, and the process of blood collection often brings pain and injury to the collector. In comparison, saliva collection is simple, painless, and low-cost. The obtained genomic DNA h...

AI Technical Summary

Problems solved by technology

At present, the DNA used for detection in laboratory departments is generally extracted from anticoagulated blood, and it is not common to extract DNA from saliva for genetic testing

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