Gastric cancer detection primer probe and kit thereof

Abstract

The invention relates to a gastric cancer detection primer and a kit thereof. The kit comprises a digital PCR premix solution, a liquid drop stabilizer, an miRNAs primer probe mixed solution, and a stem-loop reverse transcription primer mixed solution for performing RNA reverse transcription; the miRNAs primer probe mixed solution comprises an upstream primer for detecting hsa-mir-20a, hsa-mir-17, hsa-miR-106b and hsa-mir-16, a probe and a general downstream primer for detecting hsa-mir-20a, hsa-mir-17, hsa-miR-106b and hsa-mir-16. The stem-loop reverse transcription primer mixed solution comprises a stem-loop reverse transcription primer for reverse transcription of hsa-mir-20a, hsa-mir-17 and hsa-miR-106b, wherein hsa-mir-20a, hsa-mir-17 and hsa-miR-106b are markers, and hsa-mir-16 is B-actin. The gastric cancer detection kit can rapidly, conveniently and accurately detect the expression level of miRNA as the gastric cancer early diagnosis marker.

Description

technical field [0001] The invention relates to a primer probe set and a detection product for detecting the expression level of gastric cancer miRNA, belonging to the field of biotechnology. Background technique [0002] microRNA is a kind of endogenous, highly conserved non-coding small molecule RNA, which is formed by the processing of single-stranded RNA precursor molecules, generally 18-23bp in length, and is first transcribed into the original miRNA under the action of RNA polymerase II during synthesis. Then it is cleaved by the Drosha enzyme of the ribonuclease III family to form the miRNA precursor, and then processed by the Dicer enzyme of the ribonuclease III family to finally form a mature miRNA. [0003] There are a large number of miRNAs in the human body. These mature miRNAs can bind to the RNA-induced silencing complex and bind to the 3' non-coding region of its target gene mRNA to regulate the translation process after transcription, thereby regulating the e...

AI Technical Summary

Problems solved by technology

The results of sequencing technology are the most reliable, which is conducive to the discovery of new miRNAs, but the sequencing method is expensive

Although the price of miRNA chip is relatively low, its repeatability and accuracy are poor, so it is only used for primary screening of known disease-related miRNAs

The Northern Blot method is complicated and has low sensitivity, so it is not suitable for high-throughput detection of clinical samples

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