Normal pressure open-type sampling system

Abstract

The invention discloses a normal pressure open-type sampling system in a matrix-free laser desorption ionization scanning imaging mass spectrometer. The normal pressure open-type sampling system comprises a nanosecond laser optical path unit, a sample scanning platform unit and a mass spectrometer sampling unit. The nanosecond laser optical path unit comprises a middle band photo parameter oscillator, a series of apertures and reflective mirrors, an objective lens, and an optical adjustable mount. The sample scanning platform unit comprises a sample bench, a Z shaft lifting platform and a two-dimensional moving platform; the mass spectrometer sampling unit comprises a stainless steel capillary, the mass spectrometer, and a mass spectrometer orifice plate; wherein the two-dimensional moving platform and the mass spectrometer are controlled by a computer. The system can perform sample ionization under normal pressure, normal temperature and open environment, the sample pre-treatment is not required or the simple sample pre-treatment is required, micromolecules and biomacromolecules can be analysized, and the system has the advantages of rapidity and direct sampling.

Description

technical field [0001] The invention belongs to the field of sample analysis, and in particular relates to a normal-pressure open sample introduction system in a matrix-free laser analysis ionization scanning imaging mass spectrometer. The system can ionize samples under normal pressure, normal temperature and open environment, without or only needing simple sample pretreatment, and can be used to analyze small molecules and biomacromolecules, with the advantages of fast and direct sample injection. Background technique [0002] Mass spectrometry was first invented by Thomson in 1906, initially as an analytical tool for isotopes in inorganic chemistry research. At the end of the 1980s, with the advent of two soft ionization techniques, mass spectrometry developed rapidly and was widely used in the analysis of a series of biological macromolecules such as peptides, proteins, and nucleic acids, and became a proteomics Indispensable research tools in many fields, such as , tis...

AI Technical Summary

Problems solved by technology

[0004] But MALDI also has certain defects

Co-crystallization of matrix and sample is required in MALDI analysis, but the introduction of matrix will cause many problems

First of all, the selection of a suitable matrix needs to be screened and tried through a large number of experiments. At the same time, it is difficult to form a unique and uniform mixture during the co-crystallization process, which will cause hot spots in the matrix film; in addition, because the matrix used is organic Small molecules, which generate a large number of matrix adduct ions in the low mass range, severely interfering with the detection of small molecule analytes

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