Method for increasing rearing rate of black carp triploid

Abstract

The invention discloses a method for increasing a rearing rate of black carp triploid. The method comprises the following steps of preparing a tamoxifen inhibitor; preparing tamoxifen injection; treating a female parent by injecting; carrying out cold-shock and hydrostatic pressure treatment; preparing an estrogen inhibitor solution; soaking in the estrogen inhibitor solution at a normal temperature; and obtaining the triploid: taking a male tetraploid as a male parent, taking a common diploid as the female parent, and obtaining a triploid black carp system by adopting a backcross mode. According to the method disclosed by the invention, a 100% black carp triploid pure system can be obtained, the rearing rate and an emergence rate of the triploid are increased, breeding and selecting trouble are reduced; and more importantly, the method is free of pollution on germplasm of natural aquatic varieties and an ecological environment.

Description

technical field [0001] The invention relates to a method for breeding triploid herrings, in particular to a method for improving the triploid success rate of herrings. Background technique [0002] Herring is one of the four major domestic fish species cultured in freshwater in China. The sterility characteristic of triploid herring makes it show more significant advantages in terms of growth speed, stress resistance, disease resistance and meat quality. In the prior art, the sterile triploid herring is obtained by hybridization between tetraploid fish and diploid fish, but to obtain more than 98% triploid fish, then it is necessary to establish genetically stable and extremely high-purity Tetraploid fish. [0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is g...

AI Technical Summary

Problems solved by technology

[0003] In fish genetics and breeding, cold / heat shock is usually used, or methods such as hybridization are used to inhibit the first mitosis of fertilized eggs or direct heterologous chromosome doubling to prepare homologous or allotetraploid fish, and cold shock is generally Together with hydrostatic pressure treatment, it is difficult to obtain a higher-purity quadruple system, and although the heat shock method is easy to operate, it is also difficult to obtain a high-purity quadruple system; and the hybridization method has high purity, but the success rate is low , long cycle

In addition, some of the tetraploid fish prepared by the above methods are fertile and some are infertile, and there may also be gene mixing (allotetraploid), which is likely to cause biological hazards

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