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Petroleum hydrocarbon degrading strain, petroleum hydrocarbon degrading microbial agent, as well as preparation method and applications of petroleum hydrocarbon degrading microbial agent

A petroleum hydrocarbon degrading bacteria and petroleum hydrocarbon technology, which is applied in the field of petroleum pollution control, can solve problems affecting the repair effect, achieve efficient degradation, broad application prospects, and good commercial value

Active Publication Date: 2016-11-16
YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

This patented bacillus called Alicyclobium lilacearum (Acl) was found that could be used effectively at sea water with low salt concentrations without causing damage or harm on fishes living inside them. It also showed potential use in controlling contamination caused by various substances such as cruciferic acid residue left over after combating crops during production.

Problems solved by technology

Technologies aim towards finding ways to reduce or prevent harmful organism contamination (oil) during drilling operations without compromising their beneficial properties like reducing fuel consumption rates and protecting aquifests' wildlife. Current technics involve either physically removing oil films off surfaces or chemotherapy techniques involving killing cells containing heavy metals. These technical solutions require multiple steps and often result in increased costs associated with each step. Immune systems could potentially replace existing ones while maintaining their efficacy against both types of oil compounds.

Method used

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  • Petroleum hydrocarbon degrading strain, petroleum hydrocarbon degrading microbial agent, as well as preparation method and applications of petroleum hydrocarbon degrading microbial agent
  • Petroleum hydrocarbon degrading strain, petroleum hydrocarbon degrading microbial agent, as well as preparation method and applications of petroleum hydrocarbon degrading microbial agent
  • Petroleum hydrocarbon degrading strain, petroleum hydrocarbon degrading microbial agent, as well as preparation method and applications of petroleum hydrocarbon degrading microbial agent

Examples

Experimental program
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preparation example Construction

[0025] The present invention also provides a preparation method of a petroleum hydrocarbon degrading bacterial agent, comprising the following steps:

[0026] The petroleum hydrocarbon degrading strain Acinetobacter sp. LCL-1 was inserted into the petroleum degrading medium of the fermenter according to the inoculum amount of 10% by volume, and then placed in a shaking table at 150-200 rpm, at a temperature of 25- Cultivate for 3-7 days under the conditions of 35° C., salinity of 1-3.5%, and pH of 6-8 to obtain the oil-degrading bacterial agent, wherein the composition of the oil-degrading medium is 1% (w / v) KH 2 PO 4 , 1% (w / v) of K 2 HPO 4 , 1% (w / v) NH 4 NO 3 , 0.2% (w / v) MgSO 4 , 0.02% (w / v) CaCl 2 , 0.05%(w / v) FeCl 2 , 25% (w / v) NaCl and 0.5% (w / v) petroleum.

[0027] The above-mentioned petroleum-degrading bacterial agent can be used for the degradation of petroleum.

[0028] see figure 1 is the degradation rate of petroleum hydrocarbons in crude oil by Acinet...

Embodiment 1

[0036] Example 1 - Preparation of Amine Modified Polyurethane Foam:

[0037] Cut the polyurethane foam into small pieces of 1cm×1cm, take five pieces (0.5901g) and disperse them in 99mL of deionized water, add ~1.4mL of ammonia water to adjust the pH to ~10, and add 1mL of diethylenetriamine (mass) when the temperature rises to 30°C. ratio ~ 59%), stirred and reacted for 6h, and vacuum-dried to obtain graphene containing amino groups; immobilization of LCL-1: add five blocks of amine-modified polyurethane foam to 100 mL of LB medium, and connect it at a V / V ratio of 1%. After adding LCL-1 seed solution, it was placed at 30°C and 180rpm for cross-linking reaction for 72h. At this time, the fixed amount of microorganisms was about 83.55 mg / g (see figure 2 A and C).

Embodiment 2

[0038] Example 2 - Preparation of Amine-Modified Polyurethane Foam:

[0039] Cut the polyurethane foam into small pieces of 1cm×1cm, take five pieces (0.6033g) and disperse them in 50mL deionized water, add ~0.7mL ammonia water to adjust the pH to ~10, and add 50mL diethylenetriamine (mass) when the temperature rises to 30°C. ratio ~ 1.2%), stirring and reacting for 6 hours, and vacuum drying to obtain graphene containing amino groups; ② Immobilization of LCL-1: add five pieces of amine-modified polyurethane foam to 100 mL of LB medium, and connect it at a V / V ratio of 1%. After adding LCL-1 seed solution, the cross-linking reaction was carried out at 30°C and 180rpm for 72h. The microbial immobilization amount at this time was about 130.8 mg / g (see figure 2 B and D).

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Abstract

The invention provides a petroleum hydrocarbon degrading strain, namely, Acinetobacter venetianus LCL-1. According to the petroleum hydrocarbon degrading strain, the ocean bottom mud polluted by petroleum is screened to obtain ocean indigenous microbes which can adapt to the complex ocean environment and efficiently degrade petroleum hydrocarbon, the commercial value is good, and the application is wide. In addition, the invention further provides a preparation method of polyurethane foam of petroleum hydrocarbon degrading bacteria; the preparation method comprises the following steps: dispersing polyurethane foam in deionized water, adjusting the pH, adding with diethylenetriamine for water bath reaction, carrying out water washing, carrying out vacuum drying, thus obtaining amine modified polyurethane foam, dispersing the amine modified polyurethane foam in an LB culture medium, inoculating the strain into the LB culture medium, carrying out cross-linking reaction, thus obtaining the polyurethane foam of the petroleum hydrocarbon degrading bacteria; the amine modified foam has cross-linking reaction with extracelluar secreta on the surfaces of the microbes, so that immobilization and apposition growth of the microbes are facilitated, the immobilized microbes can be protected from being washed away by seawater, so that the recycling is facilitated.

Description

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Claims

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Application Information

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Owner YANTAI INST OF COASTAL ZONE RES CHINESE ACAD OF SCI
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