Aralia chinensis bud rapid propagation method

An oolong, fast technology, applied in the field of plant biology, can solve the problems of low seedling survival rate and small number of seedlings, and achieve the effect of good callus quality, low deformity rate and developed root system

Active Publication Date: 2016-11-16
WUHAN INST OF BIOENG +1
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The number of seedlings obtained by dormant axillary buds is small, limited by explant materials, and the survival rate of seedlings is low

Method used

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  • Aralia chinensis bud rapid propagation method
  • Aralia chinensis bud rapid propagation method
  • Aralia chinensis bud rapid propagation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] [Example 1] Utilizing the young leaves and tender stems of wulongtou to establish an efficient propagation system for wulongtou seedlings

[0020] 1. Prepare four media:

[0021] Explant dedifferentiation medium (A) MS+2, 4-D 1.5 mg / L+ agar 7 g / L+ sucrose 30 g / L; pH 5.8. Induce callus to differentiate into embryoid body medium (B) MS+6-BA 0.5 mg / L+NAA 0.4 mg / L+agar 7 g / L+sucrose 30 g / L+activated carbon 2 g / L; pH5.8. Embryoid body differentiation into seedling medium (C) MS+ZT 1 mg / L+NAA 0.05 mg / L+agar 7g / L+sucrose 30 g / L+activated carbon 2 g / L; pH5.8. Rooting and strong seedling medium (D) 1 / 2MS+NAA 0.1 mg / L+agar 7g / L+sucrose 30 g / L+activated carbon 2 g / L; pH5.8.

[0022] 2. Prepare explants: Prepare healthy biennial young leaves and tender stems that have just germinated in early spring and are ready for use.

[0023] 3. Explants to induce callus:

[0024] Preparation of explant dedifferentiation medium (take one liter as an example): Take about 900 ml of distilled...

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Abstract

The invention belongs to the technical field of plant biotechnology. Tender stem and young leaves of aralia chinensis bud are dedifferentiated, callus differentiates a great deal of embryoid, the embryoid efficiently differentiates to form seedlings, and rootage and seedling strengthening are carried out. The process includes the steps that a dedifferentiation culture medium is inoculated with an aralia chinensis bud explants, the callus induction rate is 100%, the aralia chinensis bud explants are transferred to a culture medium for inducing the callus to differentiate the embryoid, three times of subculturing is carried out, and the propagation coefficient of propagating third-time embryoid through the embryoid reaches 11.337; the embryoid is transferred to a culture medium for differentiating the embryoid into seedlings and is cultured for 60 days, and the seedling differentiation multiple is 3.889; the seedlings are transferred to a culture medium for rootage and seedling strengthening and are cultured for 45 days, the rooting rate is 98.6%, the average root length is 11.5 cm, and the average number of main roots is 3.2. The culture period of the method is short, few materials are used, wild resources can be effectively protected, the culture medium cost and labor cost are saved, and the method is suitable for planting aralia chinensis bud on a large scale.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a method for rapid propagation of oolong head. Background technique [0002] Oolong head, also known as "thorn dragon tooth", "wooden dragon head", "tree head vegetable", etc., is a perennial deciduous shrub or small tree of the genus Araliaceae. It is a dual-purpose medicine and vegetable produced in mountain forest areas. Rare wild vegetables. The fresh buds of oolong head are rich in nutrients, rich in various amino acids needed by the human body and a large amount of calcium, phosphorus, iron, zinc, magnesium, sodium and other mineral elements. The dried root bark, stem bark and leaves of oolong head can be used as medicine, which has high medicinal value. The main active ingredient is saponin. It has the effects of invigorating qi, promoting blood circulation, relieving pain, dispelling wind, promoting dampness, diuresis and reducing swelling. Long-term consumption can ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/005A01H4/008
Inventor 方中明李树忠
Owner WUHAN INST OF BIOENG
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