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Multispectral up-conversion nanoparticle-based immunochromatographic test paper for rapidly detecting multiple pesticide residues and preparation method for immunochromatographic test paper

An immunochromatographic test paper and pesticide multi-residue technology, applied in the field of chemical identification, to achieve the effect of narrow absorption and emission bands, low toxicity and good photostability

Inactive Publication Date: 2016-10-12
DALIAN NATIONALITIES UNIVERSITY
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Problems solved by technology

However, the use of up-conversion nano-fluorescent materials as markers, combined with immunochromatography for the analysis of small molecule pesticide residues has not been reported

Method used

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  • Multispectral up-conversion nanoparticle-based immunochromatographic test paper for rapidly detecting multiple pesticide residues and preparation method for immunochromatographic test paper
  • Multispectral up-conversion nanoparticle-based immunochromatographic test paper for rapidly detecting multiple pesticide residues and preparation method for immunochromatographic test paper
  • Multispectral up-conversion nanoparticle-based immunochromatographic test paper for rapidly detecting multiple pesticide residues and preparation method for immunochromatographic test paper

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Embodiment Construction

[0048] In recent years, due to the optical properties of rare earth up-conversion nanomaterials, their applications in the biological field have a lot of untapped potential. β-NaYF 4 :Yb 3+ ,Er 3+ (Tm 3+ ) is the material with the highest up-conversion efficiency found so far, so it has attracted extensive attention in biological detection and labeling. However, since the prepared rare earth up-conversion nanomaterials are oil-soluble materials, it is necessary to use KMnO 4 / NaIO 4 Convert oil-soluble nanoparticles to water-soluble nanoparticles. In this paper, green β-NaYF coated with oleic acid was prepared by high temperature pyrolysis. 4 :Yb 3+ ,Er 3+ Up-conversion nanoparticles, PEI-modified water-soluble red β-NaY(Gd)F prepared by hydrothermal synthesis 4 :Yb 3+ ,Er 3+ Upconverting nanoparticles.

[0049] 1. Preparation of green rare earth up-conversion nanomaterials

[0050] (1) Experimental instruments and experimental reagents

[0051] Table 1 List of e...

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Abstract

The invention relates to multispectral up-conversion nanoparticle-based immunochromatographic test paper for rapidly detecting multiple pesticide residues and a preparation method for the immunochromatographic test paper, and belongs to the technical field of chemical analysis. The pesticide test paper comprises a test paper board, a sample dropwise-addition unit, a detection unit and a sample recovery unit, wherein the sample dropwise-addition unit, the detection unit and the sample recovery unit are all connected to the test paper board; the detection unit is positioned in middle of the test paper board, and comprises an antigen coating buffer and confining liquid. The immunochromatographic test paper and the preparation method for the same have the beneficial effects that the test paper has the characteristics of rapidness, convenience, specificity, sensitivity and low cost, particularly has the characteristics of rapidness and convenience, is particularly suitable for grassroots units, field operating personnel, large-batch short-time detection and large-scale general survey and the like, has great development potential and broad application prospect, is considered as one of the most promising novel technologies for food safety detection, and becomes the main development trend of food safety detection.

Description

technical field [0001] The invention relates to the technical field of chemical identification, in particular to an immunochromatographic test paper for rapidly detecting multiple residues of pesticides based on multi-spectrum up-conversion nanoparticles and a preparation method thereof. Background technique [0002] In the early 1980s, a rapid detection technology emerged in western developed countries. The material corresponding to the binding pad is labeled, and after the action of the nitrocellulose membrane, the antibody and the antigen are specifically combined here, and this technology is called the immunochromatography test paper detection technology. These substances used for labeling all have the color or fluorescence that can be seen by the naked eye or under the excitation of ultraviolet light to see the specific binding of the antibody to the antigen. Immunochromatographic test strips are used in more and more fields because of their fastness, convenience, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/52
CPCG01N33/521G01N33/558G01N2430/10
Inventor 华瑞年国婷婷张伟于基成那立艳
Owner DALIAN NATIONALITIES UNIVERSITY
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