Preparation method of folium cortex eucommiae biologically fermented compound bacterium feed additive
A feed additive and biological fermentation technology, applied in the field of feed, can solve the problems of disease resistance of livestock and poultry, difficult to fully reflect the therapeutic effect, difficult to fully absorb the effective components of Eucommia ulmoides leaves, difficult to absorb the effective components, etc., to achieve prevention and treatment of animal intestinal diseases, Strengthens the effect of killing and inhibiting harmful bacteria and maintaining the balance of intestinal flora
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Embodiment 1
[0038] A preparation method of Eucommia ulmoides biological fermentation compound bacteria feed additive, the process is as follows:
[0039] Preparation of Eucommia ulmoides leaf extract: Weigh 500 grams of qualified fresh and dried leaves of Eucommia ulmoides, first add 500ml of water to infiltrate for 1 hour, then add 2500ml of water for the first soaking and extraction for 1 hour, the infiltration and soaking temperature are both 60°C, separate and soak For the extract, add 2000ml of water to the separated eucommia leaves for the second soaking and extraction, the soaking temperature is 60°C, the soaking time is 1 hour, and the two soaking extracts are combined to obtain.
[0040] Preparation of 10L four-stage Bacillus subtilis fermentation broth:
[0041] 1) Preparation of primary, secondary and tertiary medium:
[0042] ① Weigh 90g of peptone, 22.5g of beef extract, 15g of sodium chloride, 1.5g of ammonium sulfate, and 35ml of Eucommia leaf extract;
[0043] ② Put the ...
Embodiment 2
[0068] A preparation method of Eucommia ulmoides biological fermentation compound bacteria feed additive, the process is as follows:
[0069] Preparation of Eucommia ulmoides leaf extract: Weigh 500 grams of qualified fresh and dried leaves of Eucommia ulmoides, first add 500ml of water to infiltrate for 1 hour, then add 2500ml of water for the first soaking and extraction for 1 hour, the infiltration and soaking temperature are both 80°C, separate and soak For the extract, add 2000ml of water to the separated eucommia leaves for the second soaking and extraction, the soaking temperature is 80°C, the soaking time is 1 hour, and the two soaking extracts are combined to obtain.
[0070] Preparation of 10L four-stage Bacillus subtilis fermentation broth:
[0071] 1) Preparation of primary, secondary and tertiary medium:
[0072] ①Weigh 90g of peptone, 22.5g of beef extract, 15g of sodium chloride, 1.5g of ammonium sulfate, and 38ml of Eucommia leaf extract;
[0073] ② Put the w...
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