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Subculture method of Cornus walteri Wanger. tissue culture seedlings

A technology of subculture and tissue culture seedlings, which is applied in the field of plant tissue culture, can solve the problems of slow elongation, no high branches, and low multiplication coefficient, and achieve the goal of promoting internode elongation, increasing multiplication coefficient, and stabilizing multiplication coefficient Effect

Inactive Publication Date: 2016-10-05
BEIJING FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to solve the problems of low multiplication coefficient, slow elongation growth and no high branches in the subculture of hairy tissue cultured seedlings, optimize the medium formula, and provide a subculture of hairy hairy tissue cultured seedlings with high multiplication coefficient and elongated growth training method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] The subculture method of a kind of hairy tissue culture seedling of the present invention, comprises the following steps:

[0017] (1) Proliferation culture: Cut the aseptic tissue-cultured seedlings of Trichosanthemi that meet the subculture requirements, cut the bud clusters <1cm into small clusters on the ultra-clean workbench and inoculate them in DKW+6-BA 0.25mg / L+IBA0. In the proliferation medium of 1mg / L + sucrose 30g / L + agar powder 6.5g / L, the culture condition is 25°C, the light intensity is 1800lx, and the light is 14h per day. After one subculture proliferation cycle, clustered buds are formed, with an average proliferation coefficient of 10.27 ;

[0018] (2) Elongation culture: cut the sterile tissue cultured shoots of the hairy tree that meet the subculture requirements, and cut the strong bud branches ≥ 1 cm in the tissue cultured seedlings into ≥ 0.5 cm and containing at least one pair of buds on the ultra-clean workbench. Stem section, inoculated in DK...

Embodiment 2

[0020] The subculture method of a kind of hairy tissue culture seedling of the present invention, comprises the following steps:

[0021] (1) Proliferation culture: Cut the aseptic tissue-cultured seedlings of Trichosanthes spp. that meet the subculture requirements, cut the bud clusters <1cm into small clusters on the ultra-clean workbench and inoculate them in DKW+6-BA1mg / L+IBA0.5mg / In the proliferation medium of L + sucrose 30g / L + agar powder 6.5g / L, the culture condition is 25°C, the light intensity is 2000lx, and the light is 14h per day. After 2 subculture proliferation cycles, clustered buds are formed, with an average proliferation coefficient of 12.33;

[0022] (2) Elongation culture: cut the sterile tissue cultured shoots of the hairy tree that meet the subculture requirements, and cut the strong bud branches ≥ 1 cm in the tissue cultured seedlings into ≥ 0.5 cm and containing at least one pair of buds on the ultra-clean workbench. Stem section, inoculated in DKW+6...

Embodiment 3

[0024] The subculture method of a kind of hairy tissue culture seedling of the present invention, comprises the following steps:

[0025] (1) Proliferation culture: cut the aseptic tissue-cultured seedlings of Trichosanthemi that meet the subculture requirements, cut the bud clusters <1cm into small clusters or single buds on the ultra-clean workbench and inoculate them in DKW+6-BA 0.5mg / L In the proliferation medium of +IBA0.8mg / L+sucrose 30g / L+agar powder 6.5g / L, the culture condition is 25℃, the light intensity is 1800lx, and the light is 14h per day. Proliferation coefficient 14.18;

[0026] (2) Elongation culture: cut the sterile tissue cultured shoots of the hairy tree that meet the subculture requirements, and cut the strong bud branches ≥ 1 cm in the tissue cultured seedlings into ≥ 0.5 cm and containing at least one pair of buds on the ultra-clean workbench. Stem section, inoculated in DKW+6-BA1mg / L+NAA0.5mg / L+GA 3 In the elongation medium of 1mg / L+sucrose 30g / L+aga...

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Abstract

The invention discloses a subculture method of Cornus walteri Wanger. tissue culture seedlings. The subculture method includes: inoculating the bud tussocks, smaller than 1cm, of the sterile Cornus walteri Wanger. tissue culture seedlings satisfying subculture requirements into a proliferation culture medium, inoculating robust buds, not smaller than 1cm, of the tissue culture seedlings into an elongation culture medium, wherein the proliferation requirements of proliferation expanding propagation and high branches and robust seedlings are satisfied at the same. The subculture method has the advantages that the subculture method which is high in operability and combines proliferation and elongation growth is built by using different culture media, the average proliferation coefficient is above 10, average plant height is above 3.5cm, the tissue culture seedlings grow well, proliferation coefficient is stable after successive culture, and the subculture method is applicable to the large-scale production of the Cornus walteri Wanger. tissue culture seedlings and good in economic and social benefits.

Description

technical field [0001] The invention relates to the field of plant tissue culture, in particular to a method for subculture of hairy tissue culture seedlings. Background technique [0002] Cornus walteri Wanger. belongs to Cornus walteri Wanger, also known as car beam wood. Deciduous tree, up to 8-15m. It is distributed from Liaoning in the northeast, to Hunan in the south, to Yunnan and Guizhou in the southwest, to Jiangsu and Zhejiang in the east, and to Gansu and Qinghai in the west, among which Shandong, Henan, Shaanxi and Shanxi are relatively concentrated. The root system is developed, the adaptability is strong, and the soil requirements are not strict. It is suitable for growing on barren hills and slopes in most parts of our country. Trichosanthes is not only an excellent timber tree species, but also an excellent urban greening and soil and water conservation tree species, and it is also a good tree species for the development of forestry biodiesel. Both the pul...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00A01H4/001
Inventor 苏淑钗暴甜
Owner BEIJING FORESTRY UNIVERSITY
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