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Method for rapidly breeding seedlings by using Giantleaf Ardisia leaves

A technology of revolving fetuses and leaves is applied in the field of reproduction of revolving fetuses and seedlings, which can solve the problems of long time required for regenerating plants, cumbersome steps, long time for seed germination, etc., and achieves uniform growth, simple technological process and consistent physiological age. Effect

Active Publication Date: 2016-09-07
TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although Shi Yunping, Tang Fengluan, etc. all use tissue culture technology to breed Zoumatai seedlings, and use a small amount of materials to produce a large number of seedlings with the same traits, they all have the same disadvantages: (1) all use seeds as starting materials, so the materials Restricted by seasons; (2) The germination time of seeds is longer, and it takes a long time to obtain regenerated plants from materials, and there are many types of hormones used, and the steps are more cumbersome

Method used

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  • Method for rapidly breeding seedlings by using Giantleaf Ardisia leaves

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Leaf induced differentiation of adventitious buds

[0025] Collect the young leaves of Zoumatai, after cleaning and disinfecting, blot the water with sterile paper, then cut 50 small pieces of 0.5cm×1.0cm along the direction perpendicular to the veins, and inoculate them in the induction medium (MS+6-BA 0.8 mg / L+IBA0.3mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH value 5.8) to induce differentiation of adventitious buds, culture temperature 24-26°C, light time 10-12h / d, light intensity 20- 30μmol·m -2 ·s -1 Cultivate for about 10 days, begin to differentiate adventitious buds on the surface of the leaf incision, after cultivating for 30 days, the number of differentiated adventitious buds per leaf is 5 to 20, and the leaves that have differentiated adventitious buds are finely cut into 3 adventitious buds. Small pieces of 100 pieces were then inoculated in fresh induction medium for subculture until cluster buds were formed.

[0026] 2. Strong seedling cultivation pro...

Embodiment 2

[0037] 1. Leaf induced differentiation of adventitious buds

[0038]Collect the young leaves of Zoumatai, wash and disinfect, blot dry with sterile paper, then cut 50 small pieces of 0.7cm×1.5cm along the direction perpendicular to the veins, and inoculate them in the induction medium (MS+6-BA 1.5 mg / L+IBA0.6mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH value 5.8) to induce differentiation of adventitious buds, culture temperature 24~26℃, light time 10~12h / d, light intensity 20~ 30μmol·m -2 ·s -1 Cultivate for about 8 days, start to differentiate adventitious buds on the surface of the leaf incision, after cultivating for 30 days, the number of differentiated adventitious buds per leaf is 8 to 23, and the leaves that have differentiated adventitious buds are finely cut into 2 adventitious buds. Small pieces of 100 pieces were then inoculated in fresh induction medium for subculture until cluster buds were formed.

[0039] 2. Strong seedling cultivation process

[0040] Cut the...

Embodiment 3

[0050] 1. Leaf induced differentiation of adventitious buds

[0051] Collect the young leaves of Zoumatai, after cleaning and disinfecting, blot the water with sterile paper, then cut 50 small pieces of 0.8cm×1.8cm along the direction perpendicular to the veins, and inoculate them in the induction medium (MS+6-BA 2.0 mg / L+IBA 1.0mg / L+sucrose 30g / L+carrageenan 6.5g / L, pH value 5.8) to induce differentiation of adventitious buds, culture temperature 24~26℃, light time 10~12h / d, light intensity 20~ 30μmol·m -2 ·s -1 Cultivate for about 8 days, begin to differentiate adventitious buds on the surface of the leaf incision, after cultivating for 30 days, the number of differentiated adventitious buds per leaf is 8 to 25, and the leaves that have differentiated adventitious buds are finely cut into 2 adventitious buds. Small pieces of 100 pieces were then inoculated in fresh induction medium for subculture until cluster buds were formed.

[0052] 2. Strong seedling cultivation proc...

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Abstract

The invention belongs to the technical field of plant cultivation, and relates to a method for rapidly breeding seedlings by using Giantleaf Ardisia leaves. The method comprises the steps of leaf induced differentiation of adventitious buds, adventitious bud subculture multiplication, seedling strengthening, rooting induction, transplantation and management. The method has simple process flow, and allows a large amount of seedlings used for production and cultivation to be obtained in a short time through tissue culture adopting Giantleaf Ardisia leaves as an explant, the obtained seedlings have consistent physiologic age, tidily grow and are suitable for factory seedling growth and large scale cultivation, and the method provides technical support for development of the artificial plantation industry of Giantleaf Ardisia.

Description

technical field [0001] The invention belongs to the technical field of plant cultivation, and relates to a breeding method of Zoumatai seedlings, in particular to a process of using Zoumatai leaves to directly induce differentiation of adventitious buds, through the adventitious buds subculture multiplication, strong seedlings, rooting induction, transplanting and other processes , to obtain a large number of seedling propagation methods. Background technique [0002] Zoumatai (Ardisia gigantifolia stapf.), also known as big-leaf Zijinniu, is a plant of the genus Ardisia in the family Myrsinaceae. Evergreen, erect shrub, distributed in my country's Yunnan, Jiangxi, Fujian, Guangdong, Guangxi and other provinces (regions). It mostly grows in valleys below 1300m above sea level, in sparse and moist places beside streams and under dense forests. The rhizome and whole plant of Zoumatai have the functions of dispelling wind-dampness, strengthening muscles and bones, promoting b...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 符运柳王加宾黄碧兰李志英徐立李克烈
Owner TROPICAL CORP STRAIN RESOURCE INST CHINESE ACAD OF TROPICAL AGRI SCI
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