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A kind of method of splitting isavuconazole intermediate by enzymatic method

A technology for isavuconazole intermediate and enzymatic separation, which is applied in fermentation and other directions, can solve the problems of inability to crystallize and poor reproducibility, and achieve mild enzymatic separation conditions, easy operation, and high resolution yield Effect

Active Publication Date: 2019-07-12
CHENGDU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0014] However, the actual experiment found that the method has poor reproducibility and cannot be crystallized

Method used

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  • A kind of method of splitting isavuconazole intermediate by enzymatic method
  • A kind of method of splitting isavuconazole intermediate by enzymatic method
  • A kind of method of splitting isavuconazole intermediate by enzymatic method

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Add 100.0 g of racemic isavuconazole intermediate I, the compound of formula I, into a 500 ml flask, then add 400 ml of ethyl acetate, heat to 60°C and stir for 20 minutes, cool to room temperature, and filter to obtain filtrate A. Add 10g of commercially available AK226 nitrilase and 300ml of water into a 1000ml beaker, stir mechanically, slowly pour the filtrate A into the enzymolysis solution, stir slowly at 32°C, maintain a two-phase layered state, add 5% hydrogen dropwise Sodium oxide solution, control the pH value to 8-8.5. After the pH is stable and the sodium hydroxide solution is no longer consumed, the liquid is separated, the organic layer is filtered, and the solvent is evaporated under reduced pressure at 50°C to obtain the crude product of the compound (2S,3R)-intermediate I shown in formula IV. Add 60ml of methyl tert-butyl ether to the crude product of compound (2S,3R)-intermediate Ⅰ shown in formula IV, make a slurry, filter, and blow dry at 60°C to obt...

Embodiment 2

[0027] Add 1.00kg of racemic isavuconazole intermediate I, the compound of formula I, into a 5000ml flask, then add 3.0L of ethyl acetate, heat to 70°C and stir for 10 minutes, cool to room temperature, and filter to obtain filtrate A. Mix 120g of commercially available AK226 nitrilase with 2.0L of water, stir mechanically, slowly pour the filtrate A into the enzymolysis solution, stir at a slow speed at 35°C, maintain the state of two-phase separation, add saturated sodium carbonate solution dropwise, Control the pH value from 7.5 to 8.0. After the pH is stable and the sodium carbonate solution is no longer consumed, separate the layers, extract the water layer with 1.0L ethyl acetate, combine the organic layer and filter, remove the enzyme from the water layer, and evaporate the solvent under reduced pressure at 55°C to obtain the structure shown in formula IV Compound (2S,3R) - intermediate I crude product. Add 1.5L of isopropanol to the crude compound (2S,3R)-intermediate...

Embodiment 3

[0030] Add 1.00kg of racemic isavuconazole intermediate I, the compound of formula I, into a 5000ml flask, then add 5.0L of ethyl acetate, heat to 50°C and stir for 30 minutes, cool to 10°C, and filter to obtain filtrate A. Filter the recovered nitrilase stored in the buffer solution in Example 2, mix the filter cake with 3.0L water, stir mechanically, slowly pour the filtrate A into the enzymolysis solution, stir slowly at 40°C, and maintain two In the state of phase separation, a saturated 5% potassium hydroxide solution was added dropwise to control the pH value to 8.4-8.6. The structural compound (2R, 3S) shown in formula IV is monitored by HPLC-intermediate I enantiomer has been hydrolyzed and the remaining ≤0.2% (area normalization method), liquid separation, 2.0L ethyl acetate back extraction water layer, combined organic layer was filtered, the water layer was removed to recover the enzyme, and the solvent was distilled off under reduced pressure at 50°C to obtain the ...

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Abstract

Provided in the present invention is a method for the enzymatic resolution of an isavuconazole intermediate. Enantiomeric impurities in (2R,3S)-isavuconazole intermediate I are hydrolyzed to (2S,3S)-carboxylic acid by-product by nitrilase, and the products are separated and purified to obtain the optically pure (2S,3R)-isavuconazole intermediate I.

Description

technical field [0001] The invention relates to a method for enzymatically preparing an isavuconazole intermediate. Background technique [0002] Isavuconazole is a new triazole broad-spectrum antifungal drug approved by the US FDA in 2015. Studies have shown that isavuconazole has a broad-spectrum antifungal effect, including yeasts (such as Candida) and molds (such as Aspergillus, Mucor). [0003] Drugs that are clinically required to have optical isomers must be marketed in an optically pure form. Isavuconazole has two optical palmar centers. Therefore, the preparation of the compound ((2S,3R)-intermediate I, chirally pure) with the structure shown in formula IV is the key and difficult point in the preparation process of isavuconazole. [0004] [0005] Song Tingting et al. ("Research Progress of Antifungal Drug Isavuconazole") reviewed the construction of the chiral center of isavuconazole. The existing technologies for the chiral construction of isavuconazole inc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P41/00C12P17/10
CPCC12P17/10C12P41/00
Inventor 杜琳刘强邓道敏董强
Owner CHENGDU NORMAL UNIV
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