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Solid enzyme type time-temperature indicator

A technology of temperature indicators and enzymes, applied in thermometers, thermometers with physical/chemical changes, instruments, etc., can solve the problem of less TTI, etc., and achieve the effects of low cost, stable reaction, and convenient operation

Active Publication Date: 2016-08-17
JIANGNAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there is no relatively mature commercial TTI in my country, and scientific research institutions have relatively few researches on TTI in foreign countries, mainly focusing on electronic TTI, microbial TTI and enzyme TTI.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Preparation of discolored starch: Take 10g of soluble starch, add 5ml of 0.1mol / L iodine solution, mix evenly and put it in an oven at 54°C to dry until the final water content is less than 2%, crush it with a pulverizer to obtain discolored starch .

[0015] (2) Microencapsulation of glucoamylase: take 0.75g of chitosan, dissolve it in 100ml of 0.5% acetic acid solution, and then add 2.22g of calcium chloride to obtain a chitosan mixed solution for later use. Take 2g of sodium alginate, prepare 2% sodium alginate solution, add 0.1g of solid glucoamylase after cooling off, drop into the chitosan mixed solution with a syringe, put the obtained rubber beads into an oven at 37°C, dry them and crush them.

[0016] (3) Preparation of agar cover plate: take 3g of agar powder, prepare 3% agar solution, pour it into a container of a certain shape while it is hot, put it in the refrigerator for later use after cooling and solidifying.

[0017] (4) Production of indicator: p...

Embodiment 2

[0019] (1) Preparation of discolored starch: Take 10g of soluble starch, add 7ml of 0.1mol / L iodine solution, mix evenly and put it in a 54°C oven to dry until the final water content is less than 2%, and pulverize it with a pulverizer to obtain discolored starch .

[0020] (2) Microencapsulation of glucoamylase: take 0.75g of chitosan, dissolve it in 100ml of 0.5% acetic acid solution, and then add 2.22g of calcium chloride to obtain a chitosan mixed solution for later use. Take 2g of sodium alginate, prepare 2% sodium alginate solution, add 0.1g of solid glucoamylase after cooling off, drop into the chitosan mixed solution with a syringe, put the obtained rubber beads into an oven at 37°C, dry them and crush them.

[0021] (3) Preparation of agar cover plate: take 3g of agar powder, prepare 3% agar solution, pour it into a container of a certain shape while it is hot, put it in the refrigerator for later use after cooling and solidifying.

[0022] (4) Production of indicato...

Embodiment 3

[0024] (1) Preparation of discolored starch: take 10g of soluble starch, add 10ml of 0.1mol / L iodine solution, mix well and put it in a 54°C oven to dry until the final water content is less than 2%, and pulverize with a pulverizer to obtain discolored starch .

[0025] (2) Microencapsulation of glucoamylase: take 0.75g of chitosan, dissolve it in 100ml of 0.5% acetic acid solution, and then add 2.22g of calcium chloride to obtain a chitosan mixed solution for later use. Take 2g of sodium alginate, prepare 2% sodium alginate solution, add 0.1g of solid glucoamylase after cooling off, drop into the chitosan mixed solution with a syringe, put the obtained rubber beads into an oven at 37°C, dry them and crush them.

[0026] (3) Preparation of agar cover plate: take 3g of agar powder, prepare 3% agar solution, pour it into a container of a certain shape while it is hot, put it in the refrigerator for later use after cooling and solidifying.

[0027] (4) Production of indicator: P...

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Abstract

The invention discloses a solid enzyme type time-temperature indicator, and the indicator employs the hydrolysis reaction of saccharifying enzyme and starch, takes iodine solution as an indicator, and displays the time and temperature accumulative effect through color change, thereby achieving a purpose of indicating the time and temperature in a storage and conveying process. Starch and iodine solution are mixed together, and then are dried and smashed after color changing. The smashed mixture is mixed with emulgator solution and saccharifying enzyme microcapsules to form mash, and then mash is disposed on a paper board in a coating manner and is dried to form a color-changing bottom layer. Agar solution is prepared and cooled to form a cover plate with a certain shape for standby use. The bottom layer is combined with the cover plate, thereby activating the indicator. The indicator is apparent in color display effect, is lower in cost, and is convenient to use.

Description

technical field [0001] The invention relates to the technical field of time-temperature indication, in particular to an indicator for indicating time and temperature by coating a substrate and activating a reaction with an agar gel cover plate. Background technique [0002] With the development of the market economy, people gradually realize the importance of relying on the cold chain to extend the shelf life of food. All perishable agricultural products must rely on the cold chain to ensure that they are purchased, processed, stored, sold, and consumed. quality and reduce loss. Their quality depends not only on their own factors, but also on the influence of their environment, especially temperature. Therefore, in order to ensure the quality of food, the cold chain has a temperature requirement, that is, "no higher than the principle", and the temperature of each link from the producer to the consumer is not higher than the set temperature. Due to some human and environme...

Claims

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Application Information

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IPC IPC(8): G01K11/12G01N21/25G01K11/16
CPCG01K11/12G01N21/25
Inventor 钱静唐园圆孟晶晶
Owner JIANGNAN UNIV
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