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Seedling growing method adopting Dendrobium devonianum seed and symbiotic fungus mixed sowing

A technology of Dendrobium dendrobium and symbiotic fungi, which is applied in the field of obtaining Dendrobium dendrobium seedlings, can solve the problems that the seeding dose is greatly affected by human factors, the seeds and bacterial agents are easily washed by rain, and the liquid bacterial agents are inconvenient to carry, etc. Application and promotion, convenient storage and transportation, and the effect of improving seedling germination rate

Active Publication Date: 2016-08-03
JINGHONG HONGZHEN AGRI TECH CO LTD
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AI Technical Summary

Problems solved by technology

Seedling raising methods based on symbiotic germination technology have been reported, such as symbiotic germination and seedling growth of seedling tray seeds, but transplanting seedlings after obtaining seedlings requires a certain amount of labor and technology, which indirectly increases the cultivation cost; Seeds and fungicides are easily washed away by rain, the position of seedlings is uncertain, the seeding dose is greatly affected by human factors, and liquid fungicides are inconvenient to carry and operate.

Method used

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  • Seedling growing method adopting Dendrobium devonianum seed and symbiotic fungus mixed sowing

Examples

Experimental program
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Effect test

Embodiment 1

[0038] 1. Acquisition and preservation of tooth lobe germplasm

[0039] The seeds of Dendrobium dendrobii that are naturally strong and mature but not cracked in the field are collected and preserved for future use. Dendrobium dendrobium seeds used in this example were collected from the wild population in Longling County, Baoshan City, Yunnan Province, and the collection time was April 27, 2014.

[0040] 2. Production of fungal powder

[0041] 2.1 Production of medium: PDA liquid medium is conventionally used, with an appropriate amount. Put 50ml of each bottle into 70 bottles of 500ml Erlenmeyer flasks, wrap the mouth of the bottle with newspaper after sealing, put it into a sterilizer and sterilize it at 121°C and 115KPa for 20min, and set it aside.

[0042] 2.2 Inoculate Tulasnellasp., CGMCCNo.9551, which has been activated and cultured for 7 days, into the above-mentioned sterilized Erlenmeyer flask containing 50ml of liquid medium, and culture it at 28°C and 150rpm for...

Embodiment 2

[0056] Repeat Example 1, with the following differences:

[0057] The Longpa ancient tea garden in Yanuo Village, Jinuo Township, Jinghong City, Yunnan Province was selected as the experimental site, and the sowing experiment was carried out on May 21, 2015. A total of 105 points were seeded.

[0058] Most of the protocorms developed into seedlings 45-50 days after sowing. A total of 105 sowing points were selected for this sowing, of which 87 points had seeds germinated to form seedlings, and the group germination rate was 82.86%. A total of 1036 seedlings were obtained, and an average of 9.87 seedlings were obtained at each point.

[0059] See Table 1 for the number of sowing points, the average number of seedlings at each point and the group germination rate of the two batches of mixed sowing bags. The average population germination rate was 81.82%. A total of 1615 seedlings were obtained, and an average of 9.79 seedlings were obtained at each sowing point. Based on the s...

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Abstract

The invention discloses a seedling growing method adopting Dendrobium devonianum seed and symbiotic fungus mixed sowing. The method comprises the following steps: activating Tulasnella sp. with the preservation number represented by CGMCC No.9551, carrying out fermenting culture to prepare fungus powder, and uniformly mixing the fungus powder with Dendrobium devonianum sees and common dry mass fragments to obtain a mixed sowing bag. In the seedling growing process, the mixed sowing bag is immersed in water in advance and then is fixed on a camellia sinensis trunk through a preservative film, and the seedling rate can reach 80% at the 45-50th day. The method has the advantages low technologic cost, easy operation, high population germination rate, and fast growth and vigorousness of seedlings. The sowing bag is convenient to store and transport, and the whole process is simple and is easy to learn, so the method has good application prospect in Dendrobium devonianum emulational culture industrialization.

Description

technical field [0001] The invention belongs to the technical field of dendrobium cultivation, and in particular relates to a method for making mixed seeding bags of dendrobium dendrobium seeds and symbiotic fungi, and sowing seeds on tea trees to obtain dendrobium dendrobium seedlings. Background technique [0002] Dendrobium devonianum is a traditional Chinese medicinal material with high medicinal value, which is widely cultivated in many prefectures in Yunnan. The existing cultivation mode is mainly intensive planting, mostly in the form of "company + base + farmer", using tissue cultured seedlings as seedlings. Due to the need to build greenhouses, sprinkler irrigation systems, and land rent, the cost of planting remains high. Simultaneously, in the growth process of dendrobium seedlings, in order to promote the rapid growth of dendrobium seedlings, a large amount of chemical fertilizers and pesticides need to be used, causing product pesticide residues, heavy metal con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/02A01C1/04A01G1/00A01G1/04A01G9/10C05F11/00
CPCA01C1/02A01C1/04A01G18/00A01G22/00A01G24/00C05F11/00
Inventor 邵士成高江云黄晖刘强
Owner JINGHONG HONGZHEN AGRI TECH CO LTD
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