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Embryo-free rice freshness detecting reagent and method

A technology for germ-free rice and a detection method, which is applied in the detection reagent and detection field of germ-free rice freshness, can solve the problems of no rapid judgment method for germ-free rice storage time, etc., and achieves convenient preparation, good color rendering effect, and suitable site wide effect

Inactive Publication Date: 2016-07-27
BEIJING BEIDA MINGDE CHEM PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no quick method for judging the storage time of germ-free rice

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1) Take several samples of fresh germ-free rice (storage period less than 1 month) and old germ-free rice (storage period is about 1 year) with known storage time, shell, remove germ, and prepare several samples of rice to be tested .

[0024] 2) Take about 10mL of prepared TTC dye and add it to the plate.

[0025] 3) Take 100 grains of rice samples prepared from fresh germ-free rice, and add them to a plate, so that the rice samples can be completely soaked in the dyeing agent, and the soaking time is about 10 minutes.

[0026] 4) After soaking, pour out the excess soaking liquid, leaving only the rice sample in the plate, spread the rice sample as flat as possible without accumulation, and place it in an oven with a constant temperature of 45°C for about 15 minutes.

[0027] 5) During the constant temperature period, pay attention to the discoloration of the rice samples. If the sample is red, it is fresh rice. If the sample does not change color or has a lighter col...

Embodiment 2

[0030] 1) Take a sample of 50 grains of rice (storage period less than 1 month) and 50 grains of old rice (storage period of germless rice is about 1 year) prepared from fresh non-germ rice samples, and mix them evenly.

[0031] 2) Take about 10mL of prepared TTC dye and add it to the plate.

[0032] 3) Add the evenly mixed rice sample into the plate, so that the rice sample can be completely soaked in the dyeing agent, and the soaking time is about 10 minutes.

[0033] 4) After soaking, pour out the excess soaking liquid, leaving only the rice sample in the plate, spread the rice sample as flat as possible without accumulation, and place it in an oven with a constant temperature of 45°C for about 15 minutes.

[0034] 5) During the heat preservation period, pay attention to the discoloration of the rice samples. If the sample is red, it is fresh rice. If the sample does not change color or is lighter in color, it is old rice.

[0035] 6) Through observation and counting, it w...

Embodiment 3

[0037] 1) Sampling 50 samples of fresh rice (the storage period of non-germ rice is less than 1 month), 50 grains of fresh rice (storage period of non-germ rice is about 6 months), and 50 samples of old rice (storage period of non-germ rice) About 1 year) Take a sample of 50 grains and mix them evenly.

[0038] 2) Take about 15mL of prepared TTC dye and add it into the test cup.

[0039] 3) Add the evenly mixed rice sample into the plate, so that the rice sample can be completely soaked in the dyeing agent, and the soaking time is about 10 minutes.

[0040] 4) After soaking, pour out the excess soaking liquid, leaving only the rice sample in the plate, spread the rice sample as flat as possible without accumulation, and place it in an oven with a constant temperature of 45°C for about 15 minutes.

[0041] 5) During the heat preservation period, pay attention to the discoloration of the rice samples. If the sample is red, it is fresh rice. If the sample does not change color o...

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Abstract

The invention discloses an embryo-free rice freshness detecting reagent and method. A 1-3% red tetrazoline solution serves as the detecting reagent, and phosphate buffer with the pH value ranging from 6 to 7 serves as a solvent. According to the detecting method, rice to be detected is soaked in the red tetrazoline solution for 5-15 minutes and then taken out, heat preservation is carried out at the temperature of 45 DEG C for 5-15 minutes, and the redder the color of the rice is, the fresher the rice is; if the color of the rice does not change or is light, the rice is aged rice. The detecting reagent is good in developing effect, the developing time can be prolonged, and the detecting result stability is good; ingredients are simple, preparation is convenient, the activity of enzymes in rice endosperm can be especially detected, the judgment accuracy is high, and the judgment accuracy of the freshness of a rice sample is 95% or above. The detecting method is easy, convenient and fast to operate, no special instrument is needed, detecting cost is low, the site application range is wide, the method is very reliable and effective, and the freshness degree of the embryo-free rice can be determined qualitatively.

Description

technical field [0001] The invention relates to the technical field of rice quality detection, in particular to a detection reagent and a detection method for germ-free rice freshness. Background technique [0002] As one of the largest staple food varieties in the world, rice is also the most important staple food variety in China. During the processing of germ-free rice, multiple processes such as screening, stone removal, magnetic separation, rice milling, and polishing are required. During the processing, the husk, bran and germ of the rice are removed, so the rice is actually Endosperm of rice seeds. [0003] The quality of rice is directly related to the health of ordinary people. The taste and nutritional value of old rice are obviously worse than that of new rice. In order to increase profit margins, many unscrupulous sellers polish and wax the old rice processed by old rice and pretend it is new rice, or mix old rice with new rice for sale, which leads to the purc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
CPCG01N21/78G01N2021/775
Inventor 张冬柏吕雷郭新秋
Owner BEIJING BEIDA MINGDE CHEM PHARMA
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