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Preparation and application method of a chitosan hydrogel three-dimensional cell culture medium

A technology of three-dimensional cells and culture medium, which is applied in the field of preparation of chitosan hydrogel three-dimensional cell culture medium, can solve the problems of cost limitation, unfavorable cell treatment, negative effects on cell survival rate and utilization efficiency, etc., and achieves biocompatibility excellent effect

Active Publication Date: 2020-05-29
BEIJING UNIV OF CHEM TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Polypeptide hydrogel, as a biocompatible three-dimensional culture substrate, can better simulate the cell growth environment, but the potential risk of immune response of the outer edge polypeptide and its high cost limit its application in scientific research and practical application. Further development; Synthetic polymer physical gel has good injectability, but the drastic changes in physical conditions and long gelation time required for gelation will have a greater negative impact on the survival rate and utilization efficiency of cells. Not conducive to further cell therapy

Method used

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  • Preparation and application method of a chitosan hydrogel three-dimensional cell culture medium
  • Preparation and application method of a chitosan hydrogel three-dimensional cell culture medium
  • Preparation and application method of a chitosan hydrogel three-dimensional cell culture medium

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Take 1 chitosan cell suspension with a volume concentration of 0.03 g / mL, add 1 volume concentration of 0.015 g / mL cross-linking agent solution, mix and react at room temperature for 2 min, and obtain a hydrogel with three-dimensional distribution of cells.

Embodiment 2

[0022] Take 1 chitosan cell suspension with volume concentration of 0.03g / mL, add 1 volume concentration of 0.1g / mL cross-linking agent solution, mix and react at room temperature for 1min, and obtain a hydrogel with three-dimensional distribution of cells.

Embodiment 3

[0024] Take 3 ethylene glycol chitosan cell suspensions with a volume concentration of 0.03g / mL, add 1 volume concentration of 0.5g / mL cross-linking agent solution, and mix and react at room temperature for 0.5min to obtain a three-dimensional distribution of cells. glue.

[0025] For cell culture, the hydrogel samples prepared in the above examples can realize the transition from two-dimensional culture to three-dimensional culture, and controlling the proportion of hydrogel components to select the strength of the hydrogel can control the cell proliferation rate and the direction of stem cell differentiation.

[0026] The hydrogel has the feature of being injectable, and the hydrogel of three-dimensional cultured cells can be injected directly into the animal body to realize minimally invasive cell therapy.

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Abstract

The invention relates to preparation and use methods of a three-dimensional chitosan hydrogel cell culture medium. The cell culture medium is obtained by reacting water-soluble chitosan with a crosslinking agent. According to the three-dimensional chitosan hydrogel cell culture medium, the component safety is high, the biocompatibility is good, the hydrogel strength can be adjusted according to required cell environments, and controllable three-dimensional proliferation of in-vitro cells can be realized.

Description

technical field [0001] The invention belongs to the technical field of biological materials and hydrogel preparation, and relates to a method for preparing and using a chitosan hydrogel three-dimensional cell culture medium. Background technique [0002] In recent years, with the continuous development of biology and medicine, researchers' research on diseases is gradually deepening from the organ level to the tissue and cell level. The importance of research on cells, the building blocks of life, has become increasingly prominent. Traditional in vitro cell culture is mainly based on two-dimensional culture. Cells grow adherently on the bottom of the culture bottle or culture dish, which is quite different from the three-dimensional growth in vivo in terms of form and environment. Using a three-dimensional cell culture system can build a bridge from two-dimensional culture to three-dimensional culture in vitro, which is conducive to simulating the physiological conditions o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/09C12N5/071
CPCC12N5/0602C12N5/0693C12N2513/00C12N2533/72
Inventor 王兴李永三
Owner BEIJING UNIV OF CHEM TECH
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