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Kit and application thereof

A kit and probe technology, applied in the field of biomedicine, can solve the problems of inability to distinguish low-frequency mutations in tumor specimens with sequencing errors, unfavorable early diagnosis and postoperative monitoring of cancer, and error rate of sequencing technology.

Inactive Publication Date: 2016-07-20
天津华大基因科技有限公司 +1
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Problems solved by technology

Nowadays, high-throughput sequencing technology has been effectively applied in tumor detection, but due to the low content of free plasma DNA in the early stage of tumor onset, and the sequencing technology itself has a certain error rate, traditional sequencing methods will not be able to distinguish sequencing errors and low-frequency mutations in tumor specimens, which are not conducive to early diagnosis and postoperative monitoring of these cancers

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  • Kit and application thereof
  • Kit and application thereof

Examples

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Embodiment

[0058] The following specific implementation process takes the peripheral blood plasma of the above-mentioned subjects as the research object, and the contents are as follows:

[0059] (1) Separation of peripheral blood samples

[0060] 1. Collect 1-2 tubes (5mL / tube) of peripheral blood from the subject into EDTA anticoagulant tubes, gently invert up and down (to prevent cell rupture) 6-8 times and mix well, and perform the following within 4-6 hours on the day of blood collection deal with;

[0061] 2. Centrifuge at 1600g for 10 minutes at 4°C. After centrifugation, divide the supernatant (plasma) into multiple 1.5mL / 2mL centrifuge tubes, and the middle layer of white blood cells cannot be absorbed during the absorption process;

[0062] 3. Centrifuge at 16,000g for 10 minutes at 4°C to remove residual cells, and transfer the supernatant (plasma) to a new 1.5mL / 2mL centrifuge tube. If the white blood cells cannot be sucked to the bottom of the tube, the required plasma afte...

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Abstract

The invention provides a kit, which comprises a probe, wherein the probe is immobilized on solid-phase substrate or the probe is free in a solution; and the probe is capable of achieving specific recognition on gene regions of at least 40 genes in 145 genes in List 1. The invention also provides application of the kit, a method for constructing a target region sequencing library, a sequencing method, a method for detecting target region variation, a device for detecting the target region variation as well as a method and a device for early screening of lung cancer. By virtue of the kit and / or the method or device provided by the invention, related gene sequences of the lung cancer can be simply and conveniently acquired with high specificity in one time, and the related gene sequences can be accurately detected and analyzed, so that a detection analysis result can be used for assisting in judging or monitoring the early screening of the lung cancer.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, to a kit and its use, more specifically, the present invention relates to a kit, the use of the kit, a method for constructing a sequencing library of a target region, a sequencing method, a A method and device for detecting variation in a target region, and a method and device for early screening of lung cancer. Background technique [0002] Tumor is one of the diseases that seriously threaten human health. Since the 1970s, the incidence and mortality of cancer in my country have been on the rise. In the 20 years to the 1990s, the cancer mortality rate increased by 29.42%, and the age-adjusted mortality rate increased by 11.56%. In 2000, the incidence of cancer was about 1.8-2 million, and the death rate was 1.4-1.5 million. The incidence of malignant tumors nationwide is 87.07 / 100,000 for the age group of 35 to 39 years old, almost doubled to 154.53 / 100,000 for the age group of...

Claims

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Application Information

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IPC IPC(8): C12N15/10C12Q1/68C12M1/00C12M1/34C40B50/06
Inventor 管彦芳常连鹏陈希蔡宇航易鑫朱红梅杨玲
Owner 天津华大基因科技有限公司
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