Kit and applications thereof

Abstract

The invention provides a kit. The kit comprises a probe, wherein the probe is fixed to a solid-phase substrate or floats in a solution, and can specifically recognize a gene area of at least 10 genes among 43 genes in the table 1. The invention further provides applications of the kit, a method for constructing a target area sequencing library, a sequencing method, a method for detecting the target area variation, a device for detecting the target area variation, as well as a method and a device for carrying out early screening on the female genital tract tumor. With the adoption of the kit and / or the methods and the devices provided by the invention, the related gene sequences of the female genital tract tumor can be easily and conveniently obtained in a high-specific manner at one time, and accurately detected and analyzed, and a detection and analysis result can be used for assisting the early screening judgment or monitoring on the female genital tract tumor.

Description

technical field [0001] The present invention relates to the field of biomedicine, specifically, to a kit and its use, more specifically, the present invention relates to a kit, the use of the kit, a method for constructing a sequencing library of a target region, a sequencing method, a A method and device for detecting variation in a target region, and a method and device for early screening of gynecological reproductive tract tumors. Background technique [0002] Tumor is one of the diseases that seriously threaten human health. Since the 1970s, the incidence and mortality of cancer in my country have been on the rise. In the 20 years to the 1990s, the cancer mortality rate increased by 29.42%, and the age-adjusted mortality rate increased by 11.56%. In 2000, the incidence of cancer was about 1.8-2 million, and the death rate was 1.4-1.5 million. The incidence of malignant tumors nationwide is 87.07 / 100,000 for the age group of 35 to 39 years old, almost doubled to 154.53 / ...

AI Technical Summary

Problems solved by technology

At present, there is no routine screening method suitable for ovarian cancer and endometrial cancer. Clinically applied cytology methods, approach ultrasound, CA-125 and other methods are not specific enough to meet early screening detection, and cannot differentiating between benign and malignant tumors

Nowadays, high-throughput sequencing technology has been effectively applied in tumor detection. However, due to the low plasma free DNA content in the early stage of gynecological reproductive tract tumors, and the sequencing technology itself has a certain error rate, traditional sequencing methods will not be able to Resolving sequencing errors and low-frequency mutations in tumor specimens is detrimental to the accuracy and sensitivity of early diagnosis of gynecologic reproductive tract tumors

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