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Lung cancer screening kit

A technology of kits and reagents, applied in the field of lung cancer screening kits, to achieve good results in clinical application prospects

Active Publication Date: 2016-07-13
WEST CHINA HOSPITAL SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently no report on the relationship between Cerberus and lung cancer

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The relationship between embodiment 1 Cerberus expression level and lung cancer

[0022] The experimental method is as follows:

[0023] 1. Clinical data

[0024] Fifteen patients with early lung cancer and 10 healthy controls were selected. The basic information is as follows:

[0025] Basic Information

lung cancer patients

healthy control

number of people

15

10

age

61.2±12.2

49.5±10.6

male ratio

10(66.7％)

6(60.0％)

[0026] 2. Detection of Cerberus expression level (quantitative detection of Cerberus content)

[0027] Using the AAH-BLG-CUST kit purchased from RayBiotech (article number: AAH-BLG-CUST), the expression level of Cerberus was detected according to the following method:

[0028] 1. Sample dialysis

[0029] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (300...

Embodiment 2

[0057] Embodiment 2 The present invention detects the composition of the kit of blood Cerberus expression level and its application method

[0058] 1. The composition of the Cerberus detection kit

[0059] Detection kit (30 persons):

[0060]

[0061] 2. How to use the Cerberus detection kit

[0062] The method of use is as follows:

[0063] 1. Sample dialysis

[0064] Extract blood from lung cancer patients and healthy controls, centrifuge after EDTA anticoagulation (1200rpm, 10min), absorb the supernatant and perform a second centrifugation (3000rpm, 15min), absorb the supernatant after the second centrifugation, namely plasma, as a test Specimens were stored at -80°C after aliquoting.

[0065] Plasma samples were dialyzed using dialysis tubing prior to biotin labeling. 100 μL of samples were taken respectively and added into dialysis tubes, and then dialyzed in 4000 mL of 1×PBS (pH=8) at 4° C. while stirring. The dialysate was changed every 3 hours, and the samples...

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Abstract

The invention discloses a lung cancer screening kit. The lung cancer screening kit comprises a reagent which is randomly selected and is used for detecting a Cerberus expression level. The invention further discloses application of the reagent for detecting the Cerberus expression level to preparation of a lung cancer screening reagent. According to the kit disclosed by the invention, risks of having the lung cancer of people to be detected can be judged through detection on the Cerberus expression level, and the kit can be used for clinical auxiliary diagnosis of the lung cancer, provides effective evidence for related treatment measures or decisions for patients, and has a good clinical application prospect.

Description

technical field [0001] The invention relates to a lung cancer screening kit. Background technique [0002] Lung cancer is one of the most common malignant tumors in the world. Its morbidity and mortality are increasing year by year. Currently, the incidence rate ranks first in the world, which seriously threatens human health and life. [0003] The etiology of lung cancer is complex, and it is generally believed that the influencing factors include: ① smoking; ② environmental pollution: such as smog, interior decoration; ③ unhealthy lifestyle: such as poor eating habits, high life pressure; Silicosis, chronic bronchitis; ⑤ Intrinsic factors in the human body: such as family inheritance, decreased immune function, endocrine dysfunction, etc. [0004] At the same time, lung cancer is a disease that is good at hiding. It often shows clinical symptoms when the disease develops to an advanced stage. 70-80% of lung cancer patients are already in the middle or late stage when they...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N33/574
CPCG01N33/57423G01N33/68
Inventor 黄燕唐怀蓉雷亚莉蒋贤雯唐靖汶李为民刘伦旭张立刘丹王业
Owner WEST CHINA HOSPITAL SICHUAN UNIV
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