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Application of SERPINB3 gene in preparing osteoarthritis diagnosis preparation

An osteoarthritis and gene technology, applied in the application field of human SERPINB3 gene in the preparation of osteoarthritis diagnostic preparations

Inactive Publication Date: 2016-07-13
北京致成生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although various drug therapies have been used to treat the disease, they are ineffective for long-term control and prevention

Method used

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  • Application of SERPINB3 gene in preparing osteoarthritis diagnosis preparation
  • Application of SERPINB3 gene in preparing osteoarthritis diagnosis preparation
  • Application of SERPINB3 gene in preparing osteoarthritis diagnosis preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0069] Example 1 High-throughput sequencing screening of differentially expressed genes

[0070] 1. Sampling

[0071] From October 2012 to December 2015, patients with osteoarthritis who were treated in the Department of Orthopedics of Peking Union Medical College Hospital were collected. A total of 10 cases were collected as the case group. A total of 4 cases were collected from patients with other diseases who were hospitalized in the Department of Orthopedics during the same period as controls. The cartilage tissue samples of all subjects were obtained, numbered and stored in a -80°C low-temperature refrigerator.

[0072] The patients in the case group were all in line with the diagnostic criteria of knee OA and underwent knee arthroplasty; the control group were patients with meniscus injury and cruciate ligament injury who underwent arthroscopic surgery. According to the 1995 American Rheumatology Association diagnostic criteria for osteoarthritis, a patient with severe ...

Embodiment 2

[0094] Example 2 Verification of SERPINB3 gene expression in osteoarthritis patients and control cartilage tissues

[0095] 1. Materials

[0096] The cartilage tissues of 42 cases of osteoarthritis patients and 8 cases of control cartilage tissues were selected and grouped and numbered. The patients in the case group were all in line with the diagnostic criteria of knee OA and underwent knee arthroplasty; the control group were patients with meniscus injury and cruciate ligament injury who underwent arthroscopic surgery.

[0097] 2. Method

[0098] 2.1 Cartilage tissue is carried out total RNA is extracted, with the extraction method of embodiment 1.

[0099] 2.2 Synthesis of cDNA by reverse transcription

[0100] use IIIReverseTranscriptase (invitrogen, Cat. No. 18080-044) was used for cDNA reverse transcription, and the experimental operation was carried out according to the product manual. The specific operation was as follows:

[0101] Using a reverse transcription k...

Embodiment 3

[0119] Example 3 Papain-induced osteoarthritis model

[0120] There are many ways to establish animal models of OA, such as Hulth model, model of anterior cruciate ligament and meniscectomy, intra-articular injection of papain, ovariectomy, spontaneous formation of high-fat food feeding, joint immobilization, etc. Related studies have shown that injecting papain into the marrow joint or knee joint cavity of rabbits and guinea pigs can induce rapidly progressive osteoarthritis. Papain can decompose the proteoglycan in the cartilage matrix and promote its loss from the cartilage. The most significant changes in the cartilage of patients with osteoarthritis in the early stage are the increase of water and the decrease of proteoglycan. Therefore, the papain-induced OA model is consistent with human bone Arthritis is similar and is a better model for studying OA.

[0121] Experimental animals: 30 healthy male New Zealand white rabbits, 6 months old, weighing about 2kg. After the e...

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Abstract

The invention discloses application of an SERPINB3 gene in preparing an osteoarthritis diagnosis preparation and further discloses a product for osteoarthritis diagnosis.By means of the product, osteoarthritis can be diagnosed by detecting the expression level of the SERPINB3 gene in a tissue sample, and the product comprises a chip or a reagent kit.The invention further discloses a preparation for treating osteoarthritis.The preparation is prepared from a reagent for promoting expression of the SERPINB3 gene and a reagent for promoting an expression product of the SERPINB3 gene.The invention discloses the SERPINB3 gene related to osteoarthritis, and it is further verified that the expression of the SERPINB3 gene or the expression protein of the gene or fragments or analogues or derivatives of the gene in osteoarthritis tissue is lowered.By using the gene to detect osteoarthritis, early-stage detection can be quickly and effectively achieved, and a treatment target point and an important basis are provided for clinical application such as gene treatment and medicine treatment.

Description

technical field [0001] The invention relates to the field of biomedicine, in particular to the application of human SERPINB3 gene in preparing diagnostic preparations for osteoarthritis. Background technique [0002] Osteoarthritis (OA) is a chronic disease in which the articular cartilage, which coats the ends of bones and forms the articular surfaces of joints, gradually deteriorates over time. A number of factors have been reported to predispose patients to osteoarthritis, including genetic predisposition, obesity, accident or sports trauma, surgery, medications, and strenuous demands. Osteoarthritis is thought to begin with damage to the articular cartilage. The two most common injuries to joints are sports-related injuries and long-term "repeated use" joint injuries. The joints most often affected by osteoarthritis are the knees, hips, and hands. In most cases, osteoarthritis in the knees and hips is more disabling than hand osteoarthritis due to the essential weight...

Claims

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Application Information

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IPC IPC(8): C12Q1/68G01N33/68A61K45/00A61P19/02A61P29/00
CPCC12Q1/6883A61K45/00C12Q2600/158G01N33/6893G01N2800/105
Inventor 刘昊
Owner 北京致成生物医学科技有限公司
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