The method and application of the shuttle plasmid pmy03 in interfering with the expression of the target gene of Riemerella anatipestifer

A technology of Riemer's anatipestifer and shuttle plasmid, applied in biochemical equipment and methods, virus/phage, introduction of foreign genetic material using vectors, etc., can solve problems such as inability to knock out, and achieve good results

Active Publication Date: 2020-01-10
SICHUAN AGRI UNIV
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  • Abstract
  • Description
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  • Application Information

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Problems solved by technology

However, the essential gene of Riemerella anatipestifer cannot be knocked out by conventional knockout methods

Method used

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  • The method and application of the shuttle plasmid pmy03 in interfering with the expression of the target gene of Riemerella anatipestifer
  • The method and application of the shuttle plasmid pmy03 in interfering with the expression of the target gene of Riemerella anatipestifer
  • The method and application of the shuttle plasmid pmy03 in interfering with the expression of the target gene of Riemerella anatipestifer

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Embodiment Construction

[0023] The preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings. For the experimental methods not specified in the examples, the conventional conditions are generally followed, such as the conditions described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al.), or the conditions suggested by the manufacturer.

[0024] Utilizing the principle that the shuttle plasmid pMY03 can replicate in Riemerella anatipestifer, the target gene to be interfered was reverse-cloned into the shuttle plasmid pMY03, and then the plasmid was transferred to Riemerella anatipestifer by conjugative transfer. At this time, the antisense strand of the interfering target gene will be transcribed on the plasmid, and the transcription product will pair with the transcription product of the target gene to be interfered on the Riemeria anatipestifer genome, thereby inhibiting the expression of the target gene a...

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Abstract

The invention discloses a method of a shuttle plasmid pMY03 in interference of target gene expression of riemerella anatipestifer, and further discloses an application of the shuttle plasmid pMY03 in interference of target gene expression of riemerella anatipestifer. The method for interference of a riemerella anatipestifer RNA is built; the problem that essential gene functions of the riemerella anatipestifer cannot be researched by a gene knock-out method is solved; and the method has important significance in research of the essential gene functions of the riemerella anatipestifer.

Description

technical field [0001] The invention belongs to the field of biotechnology, and specifically relates to the application of the shuttle plasmid pMY03 in interfering with the expression of the target gene of Riemerella anatipestifer and the method for interfering with the expression of the target gene of the Riemerella anatipestifer with the shuttle plasmid pMY03. Background technique [0002] Riemerella anatipestifer is a contagious disease caused by Riemerella anatipestifer (RA), also known as duck infectious serositis. It has become one of the most common bacterial diseases that endanger the duck industry in all countries in the world. The current prevention and control methods mainly include drug prevention and vaccine prevention. However, Riemerella anatipestifer is resistant to multiple drugs, and it is difficult to find sensitive drugs for this bacteria in many areas. On the other hand, there are multiple serotypes of Riemerella anatipestifer, and there is no cross-pr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/74
CPCC12N15/74C12N2800/101
Inventor 刘马峰王梦怡岳慧贤程安春汪铭书贾仁勇朱德康陈舜杨乔吴英孙昆峰
Owner SICHUAN AGRI UNIV
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