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Method for purifying copper nano-cluster

A technology of copper nanoclusters and copper ions, which is applied in the fields of chemistry and biological sciences, can solve the problems of complicated purification, time-consuming and unsatisfactory purification effect, and achieves the effect of simple operation and low cost.

Inactive Publication Date: 2016-07-13
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these methods are relatively complicated to purify, time-consuming, and the purification effect is not ideal.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 1) Dissolve 75mg of BSA in 5ml of aqueous solution to form a clear and transparent solution;

[0023] 2), the 5mgCuSO 4 ·5H 2 O was dissolved in 1ml of water, then added to the solution obtained in step 1), and mixed evenly;

[0024] 3), under stirring, add 140ul, 1M sodium hydroxide to the solution obtained in step 2), adjust the pH value of the solution to 12, and continue stirring at a temperature of 55°C to prepare the copper nanocluster stock solution;

[0025] 4), adding 1MHAc to the copper nanocluster stock solution prepared in step 3), adjusting the pH of the copper nanocluster stock solution to 6, and promoting the generation of copper nanocluster precipitation;

[0026] 5), centrifuge and separate the copper nanocluster precipitate generated in step 4) at a speed of 10,000 rpm, and then wash the precipitate with 8 mL of deionized water for 3 times;

[0027] 6), redispersing the precipitate after washing in step 5) in sodium hydroxide solution to form a clea...

Embodiment 2

[0029] 1) Dissolving 20mg glutathione in 1ml aqueous solution to form a clear and transparent solution;

[0030] 2), the 5mgCuSO 4 ·5H 2 O was dissolved in 1ml of water, then added to the solution obtained in step 1), and mixed evenly;

[0031] 3), under stirring, add 40ul, 1M sodium hydroxide to the solution obtained in step 2), adjust the pH value of the solution to 9, and continue stirring at a temperature of 50°C to prepare the copper nanocluster stock solution;

[0032] 4), adding 0.8MHAc to the copper nanocluster stock solution prepared in step 3), adjusting the pH of the copper nanocluster stock solution to 4, and promoting the generation of copper nanocluster precipitation;

[0033] 5), centrifuge and separate the copper nanocluster precipitate generated in step 4) at a speed of 10800 rpm, and then wash the precipitate 4 times with 5 mL of deionized water;

[0034] 6), redispersing the precipitate after washing in step 5) in sodium hydroxide solution to form a clear...

Embodiment 3

[0036] 1) Dissolve 10mgBSA in 1ml aqueous solution to form a clear and transparent solution;

[0037] 2), 1mgCuSO 4 ·5H 2 O was dissolved in 0.2ml of water, then added to the solution obtained in step 1), and mixed evenly;

[0038] 3), under stirring, add 25ul, 1M sodium hydroxide to the solution obtained in step 2), adjust the pH value of the solution to 11, and continue stirring at a temperature of 58°C to prepare a copper nanocluster stock solution;

[0039] 4), add 0.9MHNO to the copper nanocluster stock solution prepared in step 3) 3 , adjust the pH of the copper nanocluster stock solution to 5, and promote the formation of copper nanocluster precipitation;

[0040] 5), centrifuge and separate the copper nano-cluster precipitate generated in step 4) at a speed of 11000 rpm, and then wash the precipitate 5 times with 7 mL of deionized water;

[0041] 6), redispersing the precipitate after washing in step 5) in sodium hydroxide solution to form a clear and transparent s...

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PUM

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Abstract

The invention discloses a method for purifying copper nano-clusters. The method comprises the following steps: 1) dissolving BSA in a solution so as to prepare a clear and transparent solution; 2) adding copper ions into the solution prepared in the step 1) according to a weight ratio of BSA to Cu<2+> of (10-40):(1-10), and uniformly mixing; 3) under the action of stirring, adding sodium hydroxide into the solution obtained in the step 2), at the same time adjusting the pH value of the solution, and stirring at 37-60 DEG C so as to obtain copper nano-cluster stoste; 4) adding HNO3 into the copper nano-cluster stoste prepared in the step 3), and adjusting the pH value of the copper nano-cluster stoste to be 3-6 so as to generate copper nano-cluster precipitate; 5) centrifuging the copper nano-cluster precipitate generated in the step 4), separating and washing; 6) dispersing the copper nano-cluster precipitate washed in the step 5) into a sodium hydroxide solution so as to prepare a clear and transparent solution, thereby obtaining the copper nano-clusters. The method has the advantages that as a copper ion-containing substance is taken as a main material, and BSA or DNA and glutathione are taken as auxiliary materials to prepare the copper nano-clusters, the method is simple and environment-friendly in operation and low in cost.

Description

technical field [0001] The invention relates to the technical fields of chemistry and biological sciences, in particular to a method for purifying copper nano-clusters. Background technique [0002] As the size of metal nanoparticles decreases, their energy level structure changes drastically, and the electronic energy levels near the Fermi level transition from a quasi-continuous state to a discrete energy level. The change of energy level structure makes metal nanoclusters have unique properties different from bulk metals; [0003] In recent years, the synthesis of water-soluble fluorescent metal nanoclusters using various template molecules and stabilizers such as proteins, DNA, and thiols as raw materials has become a research hotspot. During the synthesis process, a large number of template molecules and stabilizers need to be used, and these molecules may react with many analytes, thus causing great trouble to subsequent detection. Moreover, the inner filter effect o...

Claims

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Application Information

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IPC IPC(8): B22F9/24B22F1/00B82Y30/00B82Y40/00
CPCB82Y30/00B82Y40/00B22F9/24B22F1/07
Inventor 熊华玉王薇王升富张修华文为李玮明
Owner HUBEI UNIV
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