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A method for preparing green antibacterial and growth-promoting feed additives by two-step fermentation of aquatic protein

A feed additive and growth-promoting technology, applied in the direction of animal feed, animal feed, application, etc., can solve the problems of high cost and difficult application, and achieve the effect of low cost, reduced energy consumption and cost, and good fermentation effect.

Active Publication Date: 2019-09-17
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] Aiming at the problem that the small peptides prepared by the prior art are difficult to be widely used in the feed industry due to their high cost, the purpose of the present invention is to provide a method capable of producing small peptides at low cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Embodiment 1: the bacillus subtilis ( Bacillus subtilis )HL-2

[0034] Insert it into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150r / min for 12h to prepare Bacillus subtilis ( Bacillus subtilis ) HL-2 seed solution for use. Mince fresh Antarctic krill for later use. 15 kilograms of minced Antarctic krill and 100 kilograms of water are added to the fermenter, and 300 grams of glucose, 600 grams of calcium chloride, 600 grams of ferrous chloride and 700 grams of dipotassium hydrogen phosphate are added to the fermenter to obtain Fermentation medium; Add 8 kilograms of Bacillus subtilis ( Bacillus subtilis ) HL-2 seed liquid; the fermentation temperature is 50°C, the sterile air intake is 1.5vvm-2.5vvm, the rotation speed of the fermenter is 250rpm-350rpm, and the fermentation time is 45h; after the fermentation, the fermented liquid is filtered to remove the shrimp shell Finally, mix it with Houttuynia cordata in a ratio of 1.5:1, a...

Embodiment 2

[0035] Embodiment 2: the bacillus subtilis ( Bacillus subtilis )HL-2

[0036] Insert it into the LB liquid medium cooled after sterilization, and cultivate it at 55°C and 150r / min for 12h to prepare Bacillus subtilis ( Bacillus subtilis ) HL-2 seed solution for use. Mince fresh shrimp heads and set aside. 20 kilograms of minced shrimp heads and 100 kilograms of water are added to the fermenter, and 330 grams of glucose, 650 grams of calcium chloride, 580 grams of ferrous chloride and 750 grams of dipotassium hydrogen phosphate are added to the fermenter to obtain a fermented Medium; Add 9 kilograms of Bacillus subtilis ( Bacillus subtilis ) HL-2 seed liquid; the fermentation temperature is 55°C, the sterile air intake is 1.5vvm-2.5vvm, the rotation speed of the fermenter is 250rpm-350rpm, and the fermentation time is 40h; after the fermentation, the fermented liquid is filtered to remove the shrimp shells Finally, mix it with licorice at a ratio of 1:1, and stir well as a ...

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PUM

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Abstract

The invention discloses a method of utilizing two-step fermentation of aquatic product protein to prepare a green antibacterial growth-promoting feed additive.The method mainly includes: mincing fresh aquatic product protein; adding the minced aquatic product protein into a fermentation tank, adding nutrient and water, and mixing well to obtain a fermentation medium; inoculating Bacillus subtilis HL-2 generating high-temperature protease into the fermentation tank for liquid-state aerobic fermentation; after liquid-state aerobic fermentation is finished, filtering fermentation liquid to remove precipitate, mixing the fermentation liquid with Chinese herbal medicine, and continuing solid-state aerobic fermentation at 50-55 DEG C for 46-56h; drying, smashing and packaging a product after solid-state fermentation to obtain the green antibacterial growth-promoting feed additive.The feed additive prepared by the method is rich in small peptide and resistant to high-temperature protease, high-temperature non-starch polysaccharide, active ingredients of the Chinese herbal medicine and probiotics, has good growth-promoting performance and disease resistance and has good application prospect and great social significance.

Description

technical field [0001] The invention relates to a method for preparing a green antibacterial and growth-promoting feed additive by two-step fermentation of aquatic protein, and belongs to the technical field of feed additive preparation. Background technique [0002] Since the 1950s, antibiotics, chemically synthesized drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents have been widely used in animal feeding and feed. Antibiotics and other growth-promoting health care agents have been used in feed for a long time. It has created a revolution in the breeding industry and the feed industry, making large-scale farming develop rapidly all over the world, and the production quantity and feed conversion rate of animals have been greatly improved. increase in magnitude. However, just as all achievements of industrial civilization have its negative effects, the long-term use of non-green feed additives such as antibiotics has caused...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/26A23K10/12A23K10/30
Inventor 洪鹏志刘唤明杨萍周春霞邓楚津夏杏洲
Owner GUANGDONG OCEAN UNIVERSITY
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