Method for improving activity of polyphenol oxidase in apples by virtue of hexadecyl trimethyl ammonium bromide and dodecyl trimethyl ammonium bromide
A technology of dodecyltrimethylammonium bromide and hexadecyltrimethylammonium bromide, which is applied in the field of enzyme catalyst preparation in bioengineering, can solve the problems of enzyme activity, low PPO activity, phenolic Compound content reduction and other issues
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[0021] 1. Preparation of polyphenol oxidase crude enzyme solution:
[0022] 1. Pretreatment: Wash the apples in distilled water, dry them, and store them in a freezer at -20°C (more than 12h).
[0023] 2, the extraction of crude enzyme liquid: take by weighing 58.82g processed apples, place in 100mL phosphate buffered saline solution (0.2M, pH6.5), make a slurry with a mixer, then squeeze and filter with four layers of gauze to obtain The crude filtrate was centrifuged at 10,000 rpm for 10 min at 4°C, and the supernatant, that is, the PPO crude enzyme solution, was taken for later use.
[0024] 2. Preparation of catechol solution: prepare 300mM catechol solution in phosphate buffer solution (0.1M, pH7).
[0025] 3. Preparation of surfactant solution:
[0026] Prepare 25 mMCTAB and 60 mMDTAB solutions in deionized water, respectively.
[0027] 4. Carry out enzyme-catalyzed reaction test with catechol as substrate, and measure the activity of PPO enzyme respectively with ultr...
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