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A method for preparing green antibacterial and growth-promoting feed additive by fermenting aquatic protein

A feed additive and growth-promoting technology, applied in animal feed, animal feed, application, etc., can solve the problems of high cost and difficult application, and achieve low cost, reduced energy consumption and cost, and good fermentation effect

Active Publication Date: 2019-10-29
GUANGDONG OCEAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Aiming at the problem that the small peptides prepared by the prior art are difficult to be widely used in the feed industry due to their high cost, the purpose of the present invention is to provide a method capable of producing small peptides at low cost

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: the bacillus licheniformis capable of producing thermostable protease ( Bacillus licheniformis ) HL-3 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55 °C and 150 r / min for 12 hours to prepare Bacillus licheniformis ( Bacillus licheniformis ) HL-3 seed solution for use. Mince fresh shrimp heads and set aside. 20 kilograms of minced shrimp heads and 100 kilograms of water are added to the fermenter, and 200 grams of glucose, 550 grams of calcium chloride, 580 grams of ferrous chloride and 750 grams of dipotassium hydrogen phosphate are added to the fermenter to obtain a fermented Culture medium; Add 9 kilograms of Bacillus licheniformis ( Bacillus licheniformis ) HL-3 seed liquid; the fermentation temperature is 60°C, the sterile air intake is 1.5vvm-2.5vvm, the rotation speed of the fermenter is 250rpm-350rpm, and the fermentation time is 48h; after the fermentation, the fermented liquid is filtered to remove the sh...

Embodiment 2

[0035] Example 2: Inoculate Bacillus licheniformis HL-3, which is capable of producing thermostable protease, preserved on a slant into LB liquid medium cooled after sterilization, and cultivated at 55 °C and 150 r / min for 12 hours to prepare a lichen Bacillus ( Bacillus licheniformis ) HL-3 seed solution for use. The fresh tilapia leftovers are minced and set aside. 18 kg of ground tilapia offals and 100 kg of water were added to the fermenter, and 100 g of glucose, 520 g of calcium chloride, 550 g of ferrous chloride and 660 g of dipotassium hydrogen phosphate were added to the fermenter , to obtain the fermentation medium; add 8 kilograms of bacillus licheniformis to the fermentation medium in the fermenter ( Bacillus licheniformis ) HL-3 seed liquid; the fermentation temperature is 57°C, the sterile air intake is 1.5vvm-2.5vvm, the rotation speed of the fermenter is 250rpm-350rpm, and the fermentation time is 45h; after the fermentation, the fermented liquid is filtered ...

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PUM

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Abstract

The invention discloses a method for preparing a green antibacterial growth-promoting feed additive by utilizing aquatic protein fermentation. The method comprises the following main steps: mincing fresh aquatic proteins; adding the minced aquatic proteins into a fermentation tank, adding nutrients, uniformly mixing with water to be taken as a fermentation culture medium; introducing bacillus licheniformis HL-3 capable of producing high temperature-resistant proteases into the fermentation tank to perform liquid aerobic fermentation; after the end of liquid fermentation, filtering, removing and precipitating fermentation liquid, then mixing with Chinese herbal medicines and continuously performing solid aerobic fermentation for 46-56 hours at the temperature of 55-60 DEG C; drying, crushing and packaging a product obtained after solid-state fermentation and preparing into a green antibacterial growth-promoting feed additive finished product. The feed additive prepared by the method is rich in small peptide and resistant to high-temperature proteases, high-temperature non-starch polysaccharide enzymes, Chinese herbal medicine effective ingredients and probiotics and has good growth-promoting property and disease resistance, thereby having a good application prospect and social significance.

Description

technical field [0001] The invention relates to a method for preparing a green antibacterial and growth-promoting feed additive by fermentation of aquatic protein, and belongs to the technical field of feed additive preparation. Background technique [0002] Since the 1950s, antibiotics, chemically synthesized drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents have been widely used in animal feeding and feed. Antibiotics and other growth-promoting health care agents have been used in feed for a long time. It has created a revolution in the breeding industry and the feed industry, making large-scale farming develop rapidly all over the world, and the production quantity and feed conversion rate of animals have been greatly improved. increase in magnitude. However, just as all achievements of industrial civilization have its negative effects, the long-term use of non-green feed additives such as antibiotics has caused the foll...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K10/12A23K10/30A23K10/22A23K20/195
CPCY02P60/87
Inventor 洪鹏志刘唤明杨萍周春霞夏杏洲
Owner GUANGDONG OCEAN UNIVERSITY
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