Active substance of Antrodia camphorata mycelium and composition for protecting nerve cells
A technology of active substances and nerve cells, applied in the direction of fungi, etc., can solve the problems of affecting the composition of active ingredients, the source of basswood matrix is not fixed, and the availability of camphor wood, etc., to achieve the effect of inhibiting neurotoxicity and reducing the risk of pollution
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Embodiment 1
[0038] (1) The source of the organism
[0039] The bacterial strain A. camphorataCCRC-35396 used in the present invention was purchased from the Food Industry Development Institute.
[0040] (2) Fermentation of organisms
[0041] Inoculate the CCRC-35396 strain into 1L medium (1.0% glucose, 0.5% soybean powder, 0.5% digested protein, 0.01% MgSO 4 , 0.01% defoamer (KM-72antifoam, pH 4.0) in a 2-L Hinton flask, rotated and shaken at 28°C for ten days. The cultured Antrodia camphorata mycelium was transferred to a 200-L fermenter containing 120L of the above medium in a sterile room, and fermented at 28°C for twelve days.
[0042] (3) Extraction of organisms
[0043] Freeze-dry the mycelium fermentation liquid obtained after fermentation, extract the freeze-dried mycelium freeze-dried powder with 100% ethanol of 10-30 times its volume, and carry out shaking extraction with a shaker flask, at 10-250rpm Extract by shaking at 15-25°C for 24 hours, repeat the extraction several t...
Embodiment 2
[0047] Analysis of nerve cell toxicity damage and protection of nerve cells of active substances of Antrodia camphorata mycelium described in Example 1.
[0048] 1. Antagonize Amyloidβ (25-35) Induced primary cortical neuron toxicity
[0049] Take out the cerebral cortex (primary brain cortex) of the Sprague-Dawley rat that gave birth to one day, utilize 0.5mg / ml papaya enzyme (papain) to break up the cortical tissue and filter and lay before using polylysine solution (polylysine) -l-lysine) on the 24-well flat-bottomed tissue culture dish with a density of 5000cells / ml-well; after six days, each cell was properly pasted on it; finally these cells were tested with samples (described in Example 1) Antrodia camphorata mycelium active substance) to test. The test samples were dissolved in DMSO with concentrations of 10, 20, 40 and 50 mM, respectively, using dimethyl sulfoxide (DMSO) as a solvent. The concentration of DMSO in the medium (medium) was maintained at no more than 1...
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