Method for measuring pollutants by virtue of combination of three-dimensional fluorescence spectrum and PARAFAC algorithm

A three-dimensional fluorescence and pollutant technology, applied in fluorescence/phosphorescence, measurement device, material analysis by optical means, etc., can solve problems such as selectivity and sensitivity limitation, and achieve cumbersome compensation process, high sensitivity and good reproducibility. Effect

Inactive Publication Date: 2016-06-22
耿春茂
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Problems solved by technology

[0004] Moreover, when dealing with multi-component systems or systems with overlapping component fluorescence spectra, traditional fluorescence anal

Method used

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  • Method for measuring pollutants by virtue of combination of three-dimensional fluorescence spectrum and PARAFAC algorithm
  • Method for measuring pollutants by virtue of combination of three-dimensional fluorescence spectrum and PARAFAC algorithm
  • Method for measuring pollutants by virtue of combination of three-dimensional fluorescence spectrum and PARAFAC algorithm

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Embodiment 1

[0032] Select a variety of water samples with known concentrations of phenanthrene, pass through nitrogen to remove dissolved oxygen in the water samples, and filter the samples with a 0.45 μm microporous membrane. Take 4mL of the filtered sample and add it to a four-way cuvette, place it in a fluorescence spectrum analyzer with excitation and emission synchronization, set the excitation wavelength to 200-450nm, the emission wavelength to 280-550nm, the scanning distance to 5nm, and the 5nm, scanning speed 1200nm·min -1 Afterwards, the sample is scanned for all fluorescence data. Analyze the spectral data in the Matlab software equipped with PARAFAC algorithm toolbox, select the component in the result that meets the characteristic fluorescence spectrum peak position of the phenanthrene standard sample, export its corresponding X data value, and export it to the standard in the sample database In the curve, the corresponding concentration data are obtained.

Embodiment 2

[0034]Take 5g of naphthalene-contaminated soil samples with different known concentrations, ultrasonically extract them with 20mL of methanol for 30 minutes, centrifuge for 10 and recover the supernatant, repeat the above steps 3 times. Pass the recovered solution through the activated C18 extraction column, and elute with 10mL of n-hexane. After elution, blow off with nitrogen to nearly dryness, and use n-hexane to make the volume to 5mL. Take 2mL of the solution in the sample cell of the fluorescence photometer. Perform mapping of 3D fluorescence spectral scans. The instrument parameters are set as follows: the excitation wavelength is 200-450nm, the emission wavelength is 280-550nm, the scanning distance is 5nm, the slit width is 5nm, and the scanning speed is 1200nm·min -1 rear. The sample is scanned for all fluorescence data. Analyze the spectral data in the Matlab software equipped with the PARAFAC algorithm toolbox, select the component in the result that meets the ch...

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Abstract

The invention provides a method for determining pollutants by combining three-dimensional fluorescence spectroscopy with PARAFAC algorithm, especially for quickly identifying pollutants with fluorescent characteristics in water bodies, soils and other environments, such as hydrophobic organic pollutants with conjugated structures, benzene, phenanthrene, etc. , naphthalene, anthracene and its derivatives, benzopyrene, 1-naphthol, 2-naphthol and other pollutants. By establishing the sample database in advance, the correlation coefficient can reach more than 0.998, which has the advantages of high accuracy, high sensitivity, good reproducibility, easy operation, low analysis cost, etc., and realizes the rapid identification of organic pollution in water, soil and other environments. thing.

Description

technical field [0001] The invention provides a method for determining pollutants by three-dimensional fluorescence spectrum combined with PARAFAC algorithm, which relates to the field of identification of pollutant types and their contents, especially the rapid identification of organic pollutants in water bodies, soils and other environments, such as hydrophobic Sexual organic pollutants, benzene, phenanthrene, naphthalene, anthracene and its derivatives, benzopyridine, 1-naphthol, 2-naphthol and other pollutants. Background technique [0002] Fluorescence analysis method is one of the important methods of routine analysis. However, with the continuous development of analysis objects and the increasingly complex analysis tasks, traditional fluorescence analysis methods are difficult to meet the requirements. Because the traditional fluorescence emission (excitation) spectrum is only scanned at a certain excitation (emission) wavelength, and fluorescence is a function of bo...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6417
Inventor 耿春茂何小松
Owner 耿春茂
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