A strain of Burkholderia neocepacia and its application

Abstract

The invention provides a strain of Burkholderia neocepacia and its application. The bacteria is identified as Burkholderia neocepacia, named as Burkholderia cenocepacia CJ-P8, the deposit number is CGMCC NO.12119. The Burkholderia neocepacia CJ-P8 disclosed in the invention is screened from the rhizosphere soil of Radix Pseudostellariae through the flat plate confrontation method, and has a strong effect on the soil-borne pathogenic bacteria of Radix heterophylla isolated earlier—Fusarium oxysporum and Fusarium moniliforme Antagonism, and no pathogenicity to other crops in Pseudostellaria heterophylla. Simultaneously, the present invention provides the optimum culture medium for the growth of the antagonistic bacteria. The optimum formula is 1 / 4 LB+1 / 80 MS (without iron salt)+0.1wt.% brown sugar, and the fermented bacterial liquid has obvious antagonistic effect. The antagonistic strain provided by the invention can effectively prevent and control soil-borne diseases of heterophylla, and is pollution-free, ecological and safe, and has good application prospects.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to an antagonistic bacterium capable of antagonizing Pseudostellaria root rot, which can be used to overcome or alleviate the problem of continuous cropping Pseudostellaria soil-borne diseases, and belongs to the technical field of microorganisms and biological control. Background technique [0002] The problem of crop continuous cropping obstacles has existed for a long time. Studies have shown that there are varying degrees of continuous cropping obstacles in soybeans, cucumbers, celery, peppers, tomatoes, peanuts, potatoes, watermelons, and strawberries. In recent years, reports on continuous cropping obstacles of medicinal plants such as heterophylla, ginseng, rehmannia glutinosa, lily, and salvia miltiorrhiza have gradually increased. [0003] Pseudostellaria, also known as baby ginseng, is a precious Chinese medicinal material, which is mostly planted in Fujian, Guizhou, Jiangsu, Anhui, S...

AI Technical Summary

Problems solved by technology

However, obvious continuous cropping obstacles occurred during the cultivation of this medicinal material.

Pseudostellariae plants under continuous cropping often show short and weak aboveground plants, weakened photosynthesis of leaves, and are prone to withering and lodging; fibrous roots in underground parts cannot expand normally (such as figure 1 As shown), the medicinal ingredients cannot be effectively accumulated, and the medicinal quality is reduced; diseases and insect pests are rampant, and large areas often die. Usually, after harvesting each batch of Pseudostellaria heterophylla, it must be replanted on the same plot after an interval of 2 to 4 years

Continuous cropping not only caused a sharp decline in the yield and quality of heterophylla

However, when the causes and mechanism of continuous cropping obstacles are not yet clear, most farmers try to maintain production through measures such as increasing fertilizers and chemical fertilizers, spreading lime, and abusing pesticides. Not only is the effect not good, but it also increases production costs. A series of problems such as aggravating environmental pollution and the degradation of farmland ecosystem functions have caused the cultivation and production of heterophylla to fall into a vicious circle

Images