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A Lactic Acid Bacteria Efficiently Degrading Nitrite and Its Application in Pickle Salt

A technology of nitrite and lactic acid bacteria, which is applied in the field of bioengineering to achieve strong acid production and efficient degradation

Active Publication Date: 2019-06-11
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, lactic acid bacteria with strong acid-producing ability and high-efficiency degradation of nitrite are prepared by spray-drying into a viable bacterial agent, and added to edible salt to prepare pickle salt. This technology and product have not been reported yet.

Method used

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  • A Lactic Acid Bacteria Efficiently Degrading Nitrite and Its Application in Pickle Salt
  • A Lactic Acid Bacteria Efficiently Degrading Nitrite and Its Application in Pickle Salt
  • A Lactic Acid Bacteria Efficiently Degrading Nitrite and Its Application in Pickle Salt

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Embodiment 1

[0029] Screening of Strains with Strong Acid Production Ability

[0030] The lactic acid bacteria in the naturally fermented kimchi samples were enriched and cultivated by using MRS liquid medium, and the culture solution was serially diluted ten-fold, and added 2.0% (w / v) CaCO 3 The MRS culture medium was used as the separation medium to prepare pouring plates, cultured at 37°C for 48 hours, and the colony with the larger diameter of the transparent circle and the diameter of the colony was selected for isolation and purification. lactic acid. The isolated strains were inoculated into MRS liquid medium, cultured at 37°C for 48 hours, and the total acid in the fermentation liquid of each isolated strain was determined by potentiometric titration. figure 1 . Three strains with strong acid production ability were obtained, namely A1, A2 and A3.

[0031] Screening of Lactic Acid Bacteria Strains with Efficient Degradation of Nitrite

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Abstract

The invention belongs to the field of biotechnology, in particular to a lactic acid bacterium for efficiently degrading nitrite and its application in pickle salt. The classification of the lactic acid bacteria is named Lactobacillus plantarum Lactobacillus plantarum , the strain of Lactobacillus plantarum is preserved in the China Center for Type Culture Collection, the preservation number is CCTCC NO: M 2015184, and the acid production rate of the strain of Lactobacillus plantarum within 48 hours in the fermentation broth is 3.0×10 ‑2 to 4.0×10 ‑2 g / 100mL.h, the degradation rate of nitrite is 88.0‑98.0%. The lactic acid bacteria strains screened in this application have strong acid-producing ability and can efficiently degrade nitrite. They are prepared into live bacterial agents by spray drying, and added to edible salt to make pickle salt, which is used for pickling pickles and has lactic acid fermentation. The rapid and strong ability to degrade nitrite can greatly improve the safety of kimchi consumption.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, in particular to a lactic acid bacterium for efficiently degrading nitrite and its application in pickle salt. Background technique [0002] The history of my country's folk pickled pickles is very long, and many places have the traditional consumption habit of producing and eating by themselves. Domestic pickled kimchi adopts natural fermentation, that is, after the vegetables are washed and dried, they are fermented and pickled with edible salt water with a NaCl concentration of about 8% (w / v). During this period, the lactic acid bacteria attached to the vegetable ferment to produce lactic acid, and a large number of other microorganisms including some harmful bacteria participate in it. However, as the acid production of lactic acid bacteria increases, the pH value of the kimchi brine decreases, and the growth of miscellaneous bacteria will be inhibited. However, if only the lactic aci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23L27/40A23L29/00C12R1/25
CPCC12N1/20A23V2002/00C12R2001/25C12N1/205A23V2400/169A23V2250/1614A23V2250/636
Inventor 黄丹刘有晴罗惠波于华毛祥叶光斌
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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