Method for preparing compound type growth-promoting feed additive by fermenting Chinese herbal medicines
A technology for fermenting Chinese herbal medicines and feed additives, which is applied in the molding or processing of animal feed, animal feed, animal feed, etc. It can solve the problems of non-starch polysaccharidase loss, drug residue, and increase of drug-resistant strains, etc., so as to improve the growth-promoting performance , reduce energy consumption and cost, and increase the effect of degradation
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Embodiment 1
[0026] Embodiment 1: the thermostable Bacillus subtilis ( Bacillus subtilis ) HL-2 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h to prepare Bacillus subtilis ( Bacillus subtilis ) HL-2 seed solution for use. Grind Houttuynia cordata and pass through a 60-mesh sieve for later use. Weigh 100 kg of pulverized Houttuynia cordata, add 5200 g of bran, 380 g of calcium chloride, 390 g of ferrous chloride, 480 g of ammonium sulfate, 550 g of dipotassium hydrogen phosphate and 95 kg of water, mix well The fermentation culture that obtains after; Add 8 kilograms of bacillus subtilis ( Bacillus subtilis ) HL-2 seed solution, stirred and mixed evenly; the inoculated fermentation medium was divided into shallow fermentation pans that had been surface-sterilized with 75% alcohol. The thickness of the material in the shallow pan was 8 cm, and the surface of the material in the shallow pan was Cover the sterilized wet gauze, an...
Embodiment 2
[0027] Embodiment 2: the thermostable Bacillus subtilis ( Bacillus subtilis ) HL-2 was inserted into the LB liquid medium cooled after sterilization, and cultured at 55°C and 150r / min for 12h to prepare Bacillus subtilis ( Bacillus subtilis ) HL-2 seed solution for use. Glycyrrhizae and Radix Astragali are crushed and passed through a 60-mesh sieve for later use. Weigh 100 kg of crushed Chinese herbal medicine (70 kg of licorice + 30 kg of astragalus), and add 5600 g of bran, 490 g of calcium chloride, 460 g of ferrous chloride, 550 g of ammonium sulfate, and 560 g of dipotassium hydrogen phosphate and 90 kilograms of water, the fermentation culture obtained after mixing; add 9 kilograms of Bacillus subtilis ( Bacillus subtilis) HL-2 seed solution, stirred and mixed evenly; the inoculated fermentation medium was divided into shallow fermentation pans that had been surface-sterilized with 75% alcohol. The thickness of the material in the shallow pan was 6 cm, and the surface ...
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