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Method for extracting and purifying 15-hydroxy triptonide

A purification method and lactone ketone technology, applied in the field of traditional Chinese medicine research, can solve problems such as being highly toxic and failing to be widely promoted

Inactive Publication Date: 2016-06-01
FUJIAN ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Tripterygium wilfordii has the effects of promoting blood circulation and removing blood stasis, clearing away heat and detoxification, reducing swelling and dissipating stagnation, expelling wind and dampness, killing insects and stopping bleeding. It is highly toxic, and its clinical effective dose is close to the poisoning dose, which makes the application of Tripterygium wilfordii not widely popularized so far.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for extracting and purifying 15-hydroxytriptolide, comprising the following steps:

[0022] 1) Crush 10kg of Tripterygium wilfordii plant including stems and leaves, pass through a 20-mesh sieve, add 5 times its weight in ethanol and soak for 20 hours, then add 30 times its weight in ethanol as leachate to Tripterygium wilfordii, collect every 500mL Once, concentrate it to dryness, add 1mL of chloroform to dissolve it, and then use TLC to carry out qualitative detection on it, stop percolation until the test is negative, then combine and concentrate the percolation liquid to obtain ethanol concentrate;

[0023] 2) Extract the obtained concentrated ethanol solution with ethyl acetate at a volume ratio of 1:1.5, shake it 20 times during each extraction, concentrate the collected ethyl acetate solution after each extraction, and use TLC to characterize it Detection, after 5 times of extraction, it is detected that the extract is negative, the extraction is stopped...

Embodiment 2

[0029] A method for extracting and purifying 15-hydroxytriptolide, comprising the following steps:

[0030] 1) Crush 10kg of Tripterygium wilfordii including root core, root bark, stem and leaves, pass through a 20-mesh sieve, add 5 times its weight in ethanol and soak for 22 hours, then add 32 times its weight in ethanol as infiltration to Tripterygium wilfordii Collect the filtrate once every 500mL, concentrate it to dryness, add 1mL chloroform to dissolve it, and then use TLC to perform qualitative detection on it, stop percolation until the test is negative, then combine and concentrate the percolation liquid to obtain ethanol concentrate ;

[0031] 2) Extract the obtained concentrated ethanol solution with ethyl acetate at a volume ratio of 1:1.5, shake it 20 times during each extraction, concentrate the collected ethyl acetate solution after each extraction, and use TLC to characterize it Detection, after 5 times of extraction, it is detected that the extract is negativ...

Embodiment 3

[0037] A method for extracting and purifying 15-hydroxytriptolide, comprising the following steps:

[0038] 1) Crush 10kg of Tripterygium wisteria leaves, pass through a 20-mesh sieve, add 5 times its weight in ethanol and soak for 24 hours, then add 35 times its weight in ethanol to Tripterygium wilfordii as leachate, collect it once every 500mL, and concentrate it To dryness, add 1mL of chloroform to dissolve, then use TLC to perform qualitative detection on it, stop percolation until the test is negative, then combine and concentrate the percolation liquid to obtain ethanol concentrate;

[0039] 2) Extract the obtained concentrated ethanol solution with ethyl acetate at a volume ratio of 1:1.5, shake it 20 times during each extraction, concentrate the collected ethyl acetate solution after each extraction, and use TLC to characterize it Detection, after 6 times of extraction, it is detected that the extract is negative, stop the extraction, then combine and concentrate the ...

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PUM

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Abstract

The invention discloses a method for extracting and purifying 15-hydroxy triptonide. The 15-hydroxy triptonide of which the purity achieves 99 percent or higher can be extracted and obtained through the steps of taking root hearts, root barks, stems and leaves of triptolide as raw materials, crushing, soaking, percolating, extracting, silica gel column chromatography separation and purification, re-crystallizing and the like, and the yield of the 15-hydroxy triptonide is about 9*10<-5> and is improved by about 80 times in compassion with an existing literature report extraction process, so that a material basis is provided for the study on chemical structure, pharmacologic action and the like of the 15-hydroxy triptonide.

Description

technical field [0001] The invention belongs to the technical field of traditional Chinese medicine research, in particular to a method for extracting and purifying 15-hydroxytriptolide. Background technique [0002] Tripterygium ( Tripterygium willfordii Hook.f) is a plant of the genus Tripterygium wilfordii in the family Euonymus, which is used as medicine with the whole root or the xylem of the peeled root. Tripterygium wilfordii has the effects of promoting blood circulation and removing blood stasis, clearing heat and detoxification, reducing swelling and dissipating stagnation, expelling wind and dampness, killing insects and stopping bleeding. It is highly toxic, and its clinically effective dose is close to the poisoning dose, so the application of tripterygium wilfordii has not been widely promoted so far. [0003] In order to give full play to the medicinal efficacy of Tripterygium wilfordii, domestic and foreign scholars have isolated dozens of chemical componen...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J73/00
CPCC07J73/003
Inventor 林绥阙慧卿钱丽萍
Owner FUJIAN ACAD OF MEDICAL SCI
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