Mmc LppA protein-based indirect ELISA kit and use method

Abstract

The invention discloses an Mmc LppA protein-based indirect ELISA kit and a use method. The Mmc LppA protein-based indirect ELISA kit comprises the following raw materials according to the formula: an elisa plate coated with Mmc LppA protein, Mmc standard positive and standard negative serum, bethyl, a 50*PBST buffer solution, a closed buffer solution, a substrate fluid A, a substrate fluid B and a stop solution. The Mmc LppA protein-based indirect ELISA kit can be used for detecting sheep serum Mmc infection antibody and vaccine immunity antibody and supplying key techniques for early monitoring, vaccine immunity effect evaluation and related research work about goat and sheep mycoplasma pneumonia caused by Mmc, and has important significance in early finding, preventing and controlling for epidemic disease caused by the pathogeny.

Description

technical field [0001] The invention relates to an indirect ELISA kit based on MmcLppA protein and a use method thereof. Background technique [0002] Mycoplasma goat subspecies Mmc is one of the main pathogenic bacteria that cause chronic respiratory infectious diseases in goats and sheep and important diseases that affect the development of the sheep industry. 6d, up to 30~40d. Its clinical symptoms mainly include elevated body temperature, listlessness, loss of appetite, dyspnea and moaning in pain. Bacteria-carrying sheep and sick sheep are the main source of infection of the disease, the disease is mainly transmitted through contact and respiratory tract, and the fatality rate is high (EnginBalikci, 2008; Tian Qing, 2006). In 1971, Carmicheal isolated a new pathogenic Mycoplasma and named it Mycoplasma goat subspecies. The pathogen was successively found in many countries such as Syria, India, Sudan and Kenya. The disease was once prevalent in Inner Mongolia in my co...

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Problems solved by technology

Early mycoplasma pneumonia cases caused by Mycoplasma mycoplasma goat subspecies have not attracted people's attention for a long time, but in recent years, investigations have found that the mortality rate of the disease has been on the rise, causing serious economic losses to the sheep industry (Zhang Shuangxiang, 2013)

[0003] The prevention and control of Mycoplasma mycoplasma goat subspecies currently mainly relies on drug prevention, and vaccines can also be used, but the cost of prevention and control is very high, and the reason is related to the time-consuming and laborious isolation and cultivation of Mmc (Hou Xiangshan et al. , 2008)

After the application of clinical vaccines, there is currently a lack of rapid serological detection methods required for disease detection and prevention and control, especially the high-throughput detection and high-sensitivity ELISA method, and the lack of effective evaluation of immune prevention and control effects. Serious economic losses (Zhang Shuangxiang, 2012)

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