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A kind of preparation method of Lily Prunus necrotic ringspot virus pnrsv semi-quantitative detection gold standard card

A semi-quantitative detection and ring spot virus technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve high accuracy, good repeatability, and strong specificity

Active Publication Date: 2017-11-14
NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] In addition, the current prevention and control of lily virus is mainly based on killing the transmission medium - aphids. How to scientifically spray anti-lily virus agents, whether the proliferation of lily virus is inhibited after spraying anti-viral agents, and if it has been inhibited, what is the reason for the inhibition? There are no relevant reports on the extent of the problem, and the solution to these problems first depends on semi-quantitative detection; therefore, the realization of semi-quantitative detection is of great significance

Method used

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  • A kind of preparation method of Lily Prunus necrotic ringspot virus pnrsv semi-quantitative detection gold standard card
  • A kind of preparation method of Lily Prunus necrotic ringspot virus pnrsv semi-quantitative detection gold standard card

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Embodiment Construction

[0023] Such as figure 1 with figure 2 The shown gold standard card for semi-quantitative detection of PNRSV includes a gold standard card slot 1, a backing plate 12, a sample pad 8, a colloidal gold binding pad 9, a nitrocellulose membrane 10, and an absorbent filter paper 11, wherein the gold standard card slot 1 includes the upper The casing and the lower casing, the upper casing and the lower casing are connected by buckles, the upper casing is provided with a sample injection hole 2 and a reaction window 3, the sample pad 8 is placed under the sample injection hole 2, and the nitrocellulose membrane 10 is placed Below the reaction window 3, the gold standard probe is contained on the colloidal gold binding pad 9, the backing plate 12 is fixed in the gold standard card slot 1, the sample pad 8, the colloidal gold binding pad 9, the nitrocellulose membrane 10 and the water-absorbing filter paper 11 are sequentially Arranged and connected to the upper surface of the liner ...

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Abstract

The invention discloses a lily prunus necrotic ringspot virus (PNRSV) semi-quantitative detection colloidal gold card and a preparation method thereof; for improving the use efficiency and the field popularizing rate of a colloidal gold immunochromatographic detection method and achieving rapid and semi-quantitative detection of the lily viruses, the number of detection lines is increased to three, then the detection lines are coated by antibodies with known amounts and concentration difference, the colloidal gold card which can perform rapid semi-quantitative detection of PNRSV is developed, and the requirements of lilium spp. large-scale detoxification and rapid semi-quantitative detection of the lily viruses in fields are met; the colloidal gold card has the advantages of strong detection pertinence, simple operation, high accuracy, and strong sensitivity, and can accurately detect the difference of the contents of PNRSV of samples without the help of any instrument and equipment.

Description

technical field [0001] The invention relates to a gold standard card for rapid semi-quantitative detection of lily virus and a preparation method thereof, specifically referring to the development of a gold standard card capable of rapid semi-quantitative detection of Prunus necrotic ringspot virus PNRSV infecting lily by using colloidal gold immunochromatography Card preparation method. Background technique [0002] lily( Lilium spp. ) is the Liliaceae ( Liliaceae ) Lilium ( Lilium ) is a perennial monocotyledonous herbaceous plant. It is a world-renowned bulb flower with a long history of cultivation. It integrates ornamental, edible and medicinal uses. As the largest flower planting country in the world, the Netherlands has a relatively mature lily cut flower and bulb industry. ; In China, the edible and medicinal lily industry has developed well. The main production areas are in Gansu, Hunan and Hubei. The main varieties are Lanzhou lily, Longya lily (wild lily), et...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/569G01N33/558
CPCG01N33/558G01N33/56983G01N2333/08
Inventor 张玉宝王亚军刘维谢忠奎王若愚郭志鸿王乐
Owner NORTHWEST INST OF ECO ENVIRONMENT & RESOURCES CAS
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