Hepatic cell culture medium having ammonia reducing capacity and application of hepatic cell culture medium

Abstract

The invention discloses a hepatic cell culture medium having ammonia reducing capacity and application of the hepatic cell culture medium. The culture medium, on the basis of an RPMI 1640 liquid culture medium, is added with one or more of a hepatic cell growth factor, L-ornithine-alpha-ketoglutarate, sodium glutamate, potassium glutamate, arginine, insulin and an antioxidant, so that the problem that a great amount of ammonia is generated in the culture process of L-02 cells is solved and the ammonia output generated from the culture process of the L-02 cells is reduced; the obtained cell culture medium is capable of continuously reducing ammonia, and the culture medium is capable of enhancing the activity and the function of the L-02 cells and regulating the proliferation and the movement of the L-02 cells, so that the large-scale industrial production of the L-02 cells is achieved. In addition, the culture medium is simple in preparation method, easily available in raw material and is relatively strong in practicability.

Description

technical field [0001] The invention relates to a hepatocyte culture medium, in particular to a hepatocyte culture medium with the ability to reduce ammonia and an application thereof, belonging to the field of biotechnology. Background technique [0002] Cell culture medium is not only the basic material for supplying cell nutrition and promoting cell reproduction and proliferation in cultured cells, but also the living environment for the growth and reproduction of cultured cells. The commercially available synthetic medium is generally prepared with reagents with clear chemical components, which can be mainly divided into two types, one is liquid medium; the other is dry powder medium, and the medium commonly used in scientific research is mainly sterile liquid culture. RPMI1640 liquid medium is one of them, which was originally used for suspension culture and monolayer culture of human leukemia cells, and is now suitable for a large number of mammalian cells, including H...

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Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages that L-02 cells are prone to produce a large amount of ammonia under the prior art conditions and affect cell growth, provide a hepatocyte culture medium with the ability to reduce ammonia and its application, and solve the problem of L-02 cells in A large amount of ammonia is produced during the culture process, and a cell culture medium that can continuously reduce ammonia is obtained, and at the same time, it can enhance the vitality and function of L-02 cells

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