A strain of Sphingomonas paucimobilis and its application

Abstract

The invention discloses a Sphingomonas paucimobilis strain and application thereof and belongs to the technical field of bioengineering. The Sphingomonas paucimobilis strain is named Sphingomonas paucimobilis strain Y5, is collected in CCTCC (China Center for Type Culture Collection), which is located in Wuhan University, Wuhan, China, and has the collection number of CCTCC NO: M 2015523. Welan gum can be prepared from the train by fermenting under appropriate conditions through carbon sources, nitrogen sources, inorganic salts and water . The strain disclosed by the invention has relatively high sugar conversion ratio and welan gum yield and has a certain application value. The welan gum produced through fermenting the strain is high in quality and is resistant to acids and bases, a 1% aqueous solution viscosity of the welan gum reaches 3500mPa.S, and the viscosity is fundamentally unchanged at the temperature of 150 DEG C. During the fermentation of the strain, the sugar conversion ratio is relatively high and reaches 55% to 60%, and the yield is relatively high and reaches 25-32g / L (dry weight).

Description

technical field The invention belongs to the technical field of bioengineering, and in particular relates to a strain of Sphingomonas paucimobilis and its application in fermenting and producing welan gum. Background technique Welan gum (welan gum number S-130) is a microbial heteropolysaccharide synthesized by certain Alcaligenes sp. or Sphingomonas sp. One of the third-generation microbial exopolysaccharides developed after xanthan gum and gellan gum in the 1980s, its molecular weight is as high as several million. Its structural skeleton is composed of D-glucose, D-glucuronic acid, D-glucose and L-rhamnose repeating units, and the side chain is composed of a single L-mannose or L-rhamnose. The structure is shown in Figure 1 As shown; Welan gum aqueous solution has pseudoplastic fluid characteristics, and its low-concentration aqueous solution has high viscosity and good stability. Xanthan gum is 20-30°C higher. The aqueous solution of welan gum is stable to acid and al...

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Problems solved by technology

At present, only Kelco company in the United States commercially produces welan gum, and there is no report of commercial production in China, and domestic research is still in the initial stage of laboratory research

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