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afm1 fluorescent sensitizer

A fluorescence sensitizer and fluorescence intensity technology, which is applied in the field of chemical analysis, can solve the problems that the measurement time often changes with the change of temperature and cannot be used stably, and achieves the effect of good fluorescence enhancement effect, fast response and low corrosion.

Active Publication Date: 2018-06-05
SOUTHWEST UNIV
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

(Liu X.F..Wu'han, Chinese Academy of Agricultural Sciences (Wuhan: Chinese Academy of Agricultural Sciences), 2011.) concluded that 2,6-dimethyl-β-cyclodextrin makes AFM 1 The fluorescence intensity increased by 0.5 times, C.Cucci et al. (Cucci C., Mignani A.G., Dall'Asta C, etal.Chem., 2007,126(2),467-472) succinylated-β-cycloglutamate Fine (β-CD-SU) is used in the determination of trace AFM 1 , the results show that the fluorescence intensity can be enhanced by 1.5 times, but the application process and measurement speed are affected by the temperature and the medium to be measured, so it cannot be used stably, and the measurement time often changes with the change of temperature

Method used

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Embodiment Construction

[0020] Preferred embodiments of the present invention are described in detail below. For the experimental methods that do not specify specific conditions in the examples, usually follow the conventional conditions or the conditions suggested by the manufacturer.

[0021] 1. Instruments and reagents

[0022] F-2500 fluorescence spectrophotometer (Hitachi, Japan); analytical balance (precision 0.0001g, Shanghai Precision Scientific Instrument Co., Ltd. Balance Instrument Factory); ultrapure water generator (Milli-QA10System Millipore, France).

[0023] AFM 1 Standard (sigma); methanol (chromatographically pure, Tianjin Siyou Fine Chemicals Co., Ltd.); acetonitrile (chromatographically pure, Tianjin Siyou Fine Chemicals Co., Ltd.); β-cyclodextrin (β-CD), methyl- β-cyclodextrin (M-β-CD), hydroxypropyl-β-cyclodextrin (HP-β-CD), hydroxyethyl-β-cyclodextrin (HE-β-CD), sulfobutyl -β-cyclodextrin (SBE-β-CD), carboxymethyl-β-cyclodextrin (CM-β-CD), polymerized-β-cyclodextrin (β-CDP) ...

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Abstract

The invention discloses an aflatoxin M1 fluorescent sensitizer. The aflatoxin M1 fluorescent sensitizer is composed of methyl-beta-cyclodextrin and a Hg<2+> containing solution, wherein the mole ratio of methyl-beta-cyclodextrin to AFM1 is (5.8*10<5>):1; the mole ratio of Hg<2+> to AFM1 is (6.6*10<4>):1. The Hg<2+>-M-beta-CD fluorescent sensitizer has a remarkable reinforcing effect on aflatoxin M1 fluorescence and the reaction is quick. A related coefficient R of an AFM1 to-be-detected solution system can reach 0.999 or above in the concentration range of 0.01-2 [mu]g / L and the detection limit is 0.0026 [mu]g / L. The quick, sensitive and economic fluorescence reinforcing agent is expected to be directly applied to detection of AFM1 in dairy products and a new idea and a new approach are provided for rapid detection of AFM1.

Description

technical field [0001] The invention belongs to the field of chemical analysis, in particular to a method for aflatoxin M 1 Fluorescence sensitizer for fluorescence analysis. Background technique [0002] Aflatoxin M 1 (Aflatoxin M 1 , AFM 1 ) is the ingestion of aflatoxin B by mammals 1 (Aflatoxin B 1 , AFB 1 ) After the contaminated food is metabolized by liver cytochrome P450 and other related enzymes, the hydroxylated metabolites formed by phase II reaction occur, and the toxicity is second only to AFB 1 . In 1993, AFM 1 It is listed as a Class 2B carcinogen by the International Agency for Research on Cancer (IARC) of the World Health Organization (WTO), and was mentioned as a Class I carcinogen in 2002, which seriously threatens human health. AFM 1 It is mainly found in excreta such as milk and urine of mammals, usually when animals ingest AFB 1 AFM can be detected after 12-24 hours 1 . AFM 1 Pollution mainly exists in milk and dairy products, and humans, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
CPCG01N21/64
Inventor 马良张宇昊
Owner SOUTHWEST UNIV
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