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Multi-component micro-droplet microfluidic chip and its processing method

A microfluidic chip and micro-droplet technology, applied in chemical instruments and methods, laboratory utensils, laboratory containers, etc., can solve problems such as the inability to realize multi-component micro-droplets

Active Publication Date: 2017-10-03
EAST CHINA UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, this field has not been able to solve the key technical problems of micro-channel processing and chip bonding in the current micro-droplet microfluidic chip manufacturing process, and it is impossible to prepare complex multi-component micro-droplets in a microfluidic chip. Realize on

Method used

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Examples

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preparation example Construction

[0062] figure 2 It is a schematic diagram of the cross-sectional structure and droplet generation of the oil-in-water-in-oil type (O / W / O) multi-component micro-droplet microfluidic chip prepared according to the present invention. Such as figure 2 As shown, the microfluidic chip is composed of capping layer A, hydrophobic chip layer B, hydrophilic chip layer C and base layer D stacked in sequence; 1:4 for high-speed stirring and as water phase A; use three injection pumps to inject water phase A, oil phase, and water phase B into the microfluidic chip of the present invention from the corresponding solution inlet with a flow ratio of 1:5:25 Middle; in the hydrophobic chip layer B, the oil phase shears the water phase A from both sides, and the water phase A is sheared into a series of water-in-oil micro-droplets; the water-in-oil micro-droplets are formed by the hydrophobic chip layer B The micro-droplet generation chamber in the continuous phase flows into the lower hydro...

Embodiment 1

[0069] Example 1: Microfluidic chip for preparing O / W / O multi-component micro-droplets

[0070] The multi-component micro-droplet microfluidic chip is prepared from polymethyl methacrylate substrate using laser engraving combined with sol-gel surface modification and thermocompression bonding. Its structure is as follows: figure 1 shown.

[0071] The oil-in-water-in-oil type (O / W / O) multi-component micro-droplets were prepared by using the above-mentioned microfluidic chip. The preparation process is as figure 2 shown. The oil phase (n-octane) and deionized water added with water-based dye (0.1% methyl orange solution) were stirred at a high speed at a volume ratio of 1:4 (stirring speed was 25,000 rpm, stirring time was 5 minutes) and used as the water phase a. Using three syringe pumps, water phase A, oil phase (n-octane), water phase B (deionized water) are imported into the microfluidic chip from the corresponding solution with a flow ratio of 1:5:25; In the chip l...

Embodiment 2

[0072] Example 2: Preparation of O / W / O multi-component magnetically responsive micro-droplet microfluidic chip

[0073] Will Fe 3 o 4 Nanoparticles (3 g) and deionized water (5 mL) were stirred at high speed at a stirring speed of 18000 rpm for 5 minutes to obtain an aqueous phase A. The 1,6-hexanediol diacrylate solution (10 mL of 1,6-hexanediol diacrylate solution, PGPR900. 5g) As an oil phase, a glycerol solution (20 mL of deionized water, 1 g of glycerol, and 0.2 g of F-127) added with surfactant F-127 (Sigma-Aldrich Co. LLC.) was used as an aqueous phase B. Use three syringe pumps to inject the above-mentioned water phase A, oil phase, and water phase B into the microfluidic chip at a flow rate ratio of 1:4:16; in the hydrophobic chip layer, the water phase A is sheared by the oil phase into a series contains Fe 3 o 4 Micro-droplets of nanoparticles; the above-mentioned micro-droplets flow into the lower hydrophilic chip layer from the micro-droplet generation cham...

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Abstract

The invention relates to a multicomponent micro droplet microfluidic chip and a processing method thereof. The provided multicomponent micro droplet microfluidic chip is formed by a covering layer (A), a hydrophobic chip layer (B), a hydrophilic layer chip layer (C) and a base layer (D) which are stacked sequentially. The invention further provides a processing method of the multicomponent micro droplet microfluidic chip.

Description

technical field [0001] The invention belongs to the technical field of integrated chips, and relates to a multi-component micro-droplet microfluidic chip and a processing method thereof. Background technique [0002] Micro-droplets are a technology developed on microfluidic chips in recent years to manipulate tiny volumes of liquids. The continuous phase and the dispersed phase enter the chip through different inlets. In the microchannel, the dispersed phase is sheared by the continuous phase under the action of shear force into a series of discrete micro-droplets with uniform particle size. Each droplet is independently wrapped by the continuous phase, without material exchange with the outside world, forming a closed system, so it can be used as a microreactor to complete a set of chemical or biological reactions with stable reaction conditions and reliable results. [0003] At present, the preparation of multi-component micro-droplets has become a research hotspot in thi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): B01L3/00
CPCB01L3/502707B01L3/502715B01L3/50273
Inventor 白志山王炳捷赵双良宣晋古文全
Owner EAST CHINA UNIV OF SCI & TECH
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